1887

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Volume 62, Issue 1

Microsatellite typing of from clinical and environmental avian isolates Free

Abstract

Aspergillosis is one of the most common causes of death in captive birds. accounts for approximately 95 % of aspergillosis cases and is the second most frequent organism associated with avian infections. In the present study, the fungi were grown from avian clinical samples (post-mortem lung material) and environmental samples (eggs, food and litter). Microsatellite markers were used to type seven clinical avian isolates and 22 environmental isolates of . was the only species (28 % prevalence) detected in the avian clinical isolates, whereas this species ranked third (19 %) after members of the genera (39 %) and (21 %) in the environmental samples. Upon microsatellite analysis, five to eight distinct alleles were detected for each marker. The marker with the highest discriminatory power had eight alleles and a 0.852 value. The combination of all six markers yielded a 0.991 value with 25 distinct genotypes. One clinical avian isolate (lung biopsy) and one environmental isolate (egg) shared the same genotype. Microsatellite typing of grown from avian and environmental samples displayed an excellent discriminatory power and 100 % reproducibility. This study showed a clustering of clinical and environmental isolates, which were clearly separated. Based upon these results, aspergillosis in birds may be induced by a great diversity of isolates.

  • Received:
  • Accepted:
  • Published Online:
© 2013 SGM
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2013-01-01
2024-04-27
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Microsatellite typing of Aspergillus flavus from clinical and environmental avian isolates
J. Med. Microbiol. 62, 121 (2013); https://doi.org/10.1099/jmm.0.047803-0
/content/journal/jmm/10.1099/jmm.0.047803-0
/content/journal/jmm/10.1099/jmm.0.047803-0
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