1887

Abstract

Bee glue (propolis) possesses antimicrobial, anti-inflammatory, anaesthetic and immunostimulating activities. The aim of the study was to evaluate the inhibitory effect of Bulgarian propolis on growth . Activity of 30 % ethanolic extract of propolis (EEP) against 38 clinical isolates of was evaluated by using the agar-well diffusion method. Ethanol was used as a control. In addition, the effect of propolis on the growth of 26 and 18 strains was tested by the disc diffusion method. Mean diameters of growth inhibition by the agar-well diffusion method, using 30, 60 or 90 μl EEP or 30 μl ethanol per well, were 17.8, 21.2, 28.2 and 8.5 mm, respectively. EEP was significantly more active than ethanol against ( < 0.001). The results obtained by the disc diffusion method were similar. The use of moist propolis discs resulted in mean diameters of growth inhibition of 21.4 mm for and 13.6 mm for spp. Dried propolis discs exhibited antibacterial effect against 73.1 % of isolates, with a considerable zone of growth inhibition (⩾ 15 mm) in 36.4 % of isolates. Using dried propolis discs resulted in mean diameters of growth inhibition of 12.4 mm for and 11.6 mm for spp. In conclusion, Bulgarian propolis possesses considerable antibacterial activity against , and can also inhibit the growth of and . The potential of propolis in the prevention or treatment of infection is worth further extensive evaluation.

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2003-05-01
2024-03-28
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References

  1. Banskota A. H, Tezuka Y, Adnyana I. K, Ishii E, Midorikawa K, Matsushige K, Kadota S. 2001; Hepatoprotective and anti- Helicobacter pylori activities of constituents from Brazilian propolis. Phytomedicine 8:16–23 [CrossRef]
    [Google Scholar]
  2. Drago L, Mombelli B, De Vecchi E, Fassina M. C, Tocalli L, Gismondo M. R. 2000; In vitro antimicrobial activity of propolis dry extract. J Chemother 12:390–395 [CrossRef]
    [Google Scholar]
  3. Koo H, Gomes B. P, Rosalen P. L, Ambrosano G. M, Park Y. K, Cury J. A. 2000; In vitro antimicrobial activity of propolis and Arnica montana against oral pathogens. Arch Oral Biol 45:141–148 [CrossRef]
    [Google Scholar]
  4. Krol W, Scheller S, Shani J, Pietsz G, Czuba Z. 1993; Synergistic effect of ethanolic extract of propolis and antibiotics on the growth of Staphylococcus aureus . Arzneim-Forsch 43:607–609
    [Google Scholar]
  5. Kujumgiev A, Tsvetkova I, Serkedjieva Y, Bankova V, Christov R, Popov S. 1999; Antibacterial, antifungal and antiviral activity of propolis of different geographic origin. J Ethnopharmacol 64:235–240 [CrossRef]
    [Google Scholar]
  6. Megraud F. 2000; Strategies to treat patients with antibiotic resistant Helicobacter pylori . Int J Antimicrob Agents 16:507–509 [CrossRef]
    [Google Scholar]
  7. Megraud F, Broutet N. 2000; Review article: have we found the source of Helicobacter pylori ?. Aliment Pharmacol Ther 14:Suppl. 37–12
    [Google Scholar]
  8. Mirzoeva O. K, Grishanin R. N, Calder P. C. 1997; Antimicrobial action of propolis and some of its components: the effect on growth, membrane potential and motility of bacteria. Microbiol Res 152:239–246 [CrossRef]
    [Google Scholar]
  9. Velikova M, Bankova V, Sorkun K, Houcine S, Tsvetkova I, Kujumgiev A. 2000; Propolis from the Mediterranean region: chemical composition and antimicrobial activity. Z Naturforsch [C] 55:790–793
    [Google Scholar]
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