1887

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Volume 62, Issue 8

Vesicle formation as a result of interaction between polymorphonuclear neutrophils and biofilm Free

Abstract

, a major opportunistic pathogen, is a leading cause of biofilm-related infections in clinical practice. Staphylococcal biofilms are highly resistant to antibacterial medicines and immune effector cells. The main result of our work is the discovery of nano-vesicles in the supernatant of the human neutrophil– biofilm system. We also found that phospholipase C treatment causes complete destruction of these vesicles. While the addition of proteinase K led to a partial structural disorganization of the vesicles, DNase treatment did not influence the vesicle structure. These observations allowed us to conclude that phospholipids and proteins play a structure-forming role in the formation of these nano-vesicles. The vesicles demonstrated anti-biofilm activities when tested against (strains 178M and 328/5) biofilms, but were ineffective for (strains 5983/2, 5663 and 18A) biofilms.

  • Received:
  • Accepted:
  • Published Online:
© 2013 SGM
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/content/journal/jmm/10.1099/jmm.0.048967-0
2013-08-01
2024-04-18
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Vesicle formation as a result of interaction between polymorphonuclear neutrophils and Staphylococcus aureus biofilm
J. Med. Microbiol. 62, 1153 (2013); https://doi.org/10.1099/jmm.0.048967-0
/content/journal/jmm/10.1099/jmm.0.048967-0
/content/journal/jmm/10.1099/jmm.0.048967-0
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