@article{mbs:/content/journal/jmm/10.1099/jmm.0.04966-0, author = "Jain, Amita and Roy, Indranil and Gupta, Mahendra K. and Kumar, Mala and Agarwal, S. K.", title = "Prevalence of extended-spectrum β-lactamase-producing Gram-negative bacteria in septicaemic neonates in a tertiary care hospital", journal= "Journal of Medical Microbiology", year = "2003", volume = "52", number = "5", pages = "421-425", doi = "https://doi.org/10.1099/jmm.0.04966-0", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.04966-0", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", abstract = "The present study was undertaken to investigate the high incidence of multiresistant Gram-negative bacilli causing neonatal septicaemia. Samples of neonatal blood from 728 suspected cases were obtained in brain heart infusion broth with sodium polyanethol sulfonate. All Gram-negative rods isolated were subsequently subjected to routine antimicrobial susceptibility testing and tests for extended-spectrum β-lactamase (ESBL) production, as per NCCLS recommendations. ESBL was detected in 86.6 % of Klebsiella spp., 73.4 % of Enterobacter spp. and 63.6 % of Escherichia coli strains. It was also observed that 74.4–80.9 % of these ESBL producers were resistant to cefotaxime and 47.6–59.5 % were resistant to ceftazidime in routine susceptibility testing. Some ESBL producers (36.3–61.5 %) were found to be susceptible to either or both cephalosporins used in this study. It is concluded that indiscriminate use of third-generation cephalosporins may be responsible for the selection of ESBL-producing multiresistant strains in the neonatal intensive-care unit (NICU).", }