RT Journal Article SR Electronic(1) A1 Jain, Amita A1 Roy, Indranil A1 Gupta, Mahendra K. A1 Kumar, Mala A1 Agarwal, S. K.YR 2003 T1 Prevalence of extended-spectrum β-lactamase-producing Gram-negative bacteria in septicaemic neonates in a tertiary care hospital JF Journal of Medical Microbiology, VO 52 IS 5 SP 421 OP 425 DO https://doi.org/10.1099/jmm.0.04966-0 PB Microbiology Society, SN 1473-5644, AB The present study was undertaken to investigate the high incidence of multiresistant Gram-negative bacilli causing neonatal septicaemia. Samples of neonatal blood from 728 suspected cases were obtained in brain heart infusion broth with sodium polyanethol sulfonate. All Gram-negative rods isolated were subsequently subjected to routine antimicrobial susceptibility testing and tests for extended-spectrum β-lactamase (ESBL) production, as per NCCLS recommendations. ESBL was detected in 86.6 % of Klebsiella spp., 73.4 % of Enterobacter spp. and 63.6 % of Escherichia coli strains. It was also observed that 74.4–80.9 % of these ESBL producers were resistant to cefotaxime and 47.6–59.5 % were resistant to ceftazidime in routine susceptibility testing. Some ESBL producers (36.3–61.5 %) were found to be susceptible to either or both cephalosporins used in this study. It is concluded that indiscriminate use of third-generation cephalosporins may be responsible for the selection of ESBL-producing multiresistant strains in the neonatal intensive-care unit (NICU)., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.04966-0