1887

Abstract

There is increasing recognition of the emerging role of manganese regulation and acquisition in some pathogenic bacteria. Expression of the endocarditis-associated virulence factor EfaA is induced by growth in serum. It is demonstrated here that expression of the operon encoding a putative ABC-type transporter is regulated by Mn. Transcription of and EfaA production were repressed in Mn-supplemented medium. A Mn-responsive transcriptional regulator, EfaR, sharing 27 % identity with the diphtheria toxin repressor (DtxR), was identified. In the presence of Mn, EfaR protein bound to the promoter region. Analysis of the V583 genome revealed ten additional putative EfaR-binding sites, suggesting that manganese availability could have a broader regulatory role in infection. The results identify a new Mn-sensing regulator in enterococci that regulates the expression of a virulence factor implicated in enterococcal endocarditis.

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2003-02-01
2024-05-01
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References

  1. Aitchison E. J, Lambert P. A, Smith E. G, Farrell I. D. 1987; Serodiagnosis of Streptococcus faecalis endocarditis by immunoblotting of surface protein antigens. J Clin Microbiol 25:211–215
    [Google Scholar]
  2. Bartsevich V. V, Pakrasi H. B. 1996; Manganese transport in the cyanobacterium Synechocystis sp. PCC 6803.. J Biol Chem 271:26057–26061 [CrossRef]
    [Google Scholar]
  3. Gold B, Rodriguez G. M, Marras S. A. E, Pentecost M, Smith I. 2001; The Mycobacterium tuberculosis IdeR is a dual functional regulator that controls transcription of genes involved in iron acquisition, iron storage and survival in macrophages. Mol Microbiol 42:851–865
    [Google Scholar]
  4. Hill P. J, Cockayne A, Landers P, Morrissey J. A, Sims C. M, Williams P. 1998; SirR, a novel iron-dependent repressor in Staphylococcus epidermidis . Infect Immun 66:4123–4129
    [Google Scholar]
  5. Horsburgh M. J, Clements M. O, Crossley H, Ingham E, Foster S. J. 2001; PerR controls oxidative stress resistance and iron storage proteins and is required for virulence in S taphylococcus aureus . Infect Immun 69:3744–3754 [CrossRef]
    [Google Scholar]
  6. Horsburgh M. J, Wharton S. J, Cox A. G, Ingham E, Peacock S, Foster S. J. 2002; MntR modulates expression of the PerR regulon and superoxide resistance in Staphylococcus aureus through control of manganese uptake. Mol Microbiol 44:1269–1286 [CrossRef]
    [Google Scholar]
  7. Jacob A. E, Hobbs S. J. 1974; Conjugal transfer of plasmid-borne multiple antibiotic resistance in Streptococcus faecalis var. zymogenes . J Bacteriol 117:360–372
    [Google Scholar]
  8. Jakubovics N. S, Jenkinson H. F. 2001; Out of the iron age: new insights into the critical role of manganese homeostasis in bacteria. Microbiology 147:1709–1718
    [Google Scholar]
  9. Jakubovics N. S, Smith A. W, Jenkinson H. F. 2000; Expression of the virulence-related Sca (Mn2+) permease in Streptococcus gordonii is regulated by a diphtheria toxin metallorepressor-like protein ScaR. Mol Microbiol 38:140–153 [CrossRef]
    [Google Scholar]
  10. Jakubovics N. S, Smith A. W, Jenkinson H. F. 2002; Oxidative stress tolerance is manganese (Mn2+) regulated in Streptococcus gordonii . Microbiology 148:3255–3263
    [Google Scholar]
  11. Kolenbrander P. E, Andersen R. N, Baker R. A, Jenkinson H. F. 1998; The adhesion-associated sca operon in Streptococcus gordonii encodes an inducible high-affinity ABC transporter for Mn2+ uptake. J Bacteriol 180:290–295
    [Google Scholar]
  12. Krachler M, Rossipal E, Micetic-Turk D. 1999; Concentrations of trace elements in sera of newborns, young infants, and adults. Biol Trace Elem Res 68:121–135 [CrossRef]
    [Google Scholar]
  13. Landman D, Quale J. M. 1997; Management of infections due to resistant enterococci: a review of therapeutic options. J Antimicrob Chemother 40:161–170 [CrossRef]
    [Google Scholar]
  14. Lee J. H, Wang T, Ault K, Liu J, Schmitt M. P, Holmes R. K. 1997; Identification and characterization of three new promoter/operators from Corynebacterium diphtheriae that are regulated by the diphtheria toxin repressor (DtxR) and iron. Infect Immun 65:4273–4280
    [Google Scholar]
  15. Lowe A. M, Lambert P. A, Smith A. W. 1995; Cloning of an Enterococcus faecalis endocarditis antigen: homology with adhesins from some oral streptococci. Infect Immun 63:703–706
    [Google Scholar]
  16. Murray B. E. 1990; The life and times of the enterococcus. Clin Microbiol Rev 3:46–65
    [Google Scholar]
  17. Mylonakis E, Calderwood S. B. 2001; Infective endocarditis in adults. N Engl J Med 345:1318–1330 [CrossRef]
    [Google Scholar]
  18. Sambrook J, Fritsch E. F, Maniatis T. 1989 Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  19. Shorrock P. J, Lambert P. A, Aitchison E. J, Smith E. G, Farrell I. D, Gutschik E. 1990; Serological response in Enterococcus faecalis endocarditis determined by enzyme-linked immunosorbent assay. J Clin Microbiol 28:195–200
    [Google Scholar]
  20. Singh K. V, Coque T. M, Weinstock G. M, Murray B. E. 1998; In vivo testing of an Enterococcus faecalis efaA mutant and use of efaA homologs for species identification. FEMS Immunol Med Microbiol 21:323–331 [CrossRef]
    [Google Scholar]
  21. Skjold S. A, Quie P. G, Fries L. A, Barnham M, Cleary P. P. 1987; DNA fingerprinting of Streptococcus zooepidemicus (Lancefield group C) as an aid to epidemiological study. J Infect Dis 155:1145–1150 [CrossRef]
    [Google Scholar]
  22. Swartz M. N. 1994; Hospital-acquired infections: diseases with increasingly limited therapies. Proc Natl Acad Sci U S A 91:2420–2427 [CrossRef]
    [Google Scholar]
  23. Tao X, Schiering N, Zeng H. Y, Ringe D, Murphy J. R. 1994; Iron, DtxR, and the regulation of diphtheria toxin expression. Mol Microbiol 14:191–197 [CrossRef]
    [Google Scholar]
  24. Tseng H. J, Srikhanta Y, McEwan A. G, Jennings M. P. 2001; Accumulation of manganese in Neisseria gonorrhoeae correlates with resistance to oxidative killing by superoxide anion and is independent of superoxide dismutase activity. Mol Microbiol 40:1175–1186 [CrossRef]
    [Google Scholar]
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