1887

Abstract

The ability of phage-typing and I chromosomal RFLPs to conclude appropriate strain relatedness between a collection of 12 well-characterized -selected vancomycin-intermediate (VISA) strains and their seven vancomycin-susceptible parent strains is reported. Generally, no I RFLP alterations were observed in VISA strains when they were compared with their respective parent strains, and clonal relationships between isogenic strains were clearly evident. Unlike the I RFLP results, parent strains and VISA derivatives generally did not share similar phage-typing profiles. Depending on the phage set investigated, some VISA strains even became untypable by this method. Loss of phage infectivity is probably due to cell wall (phage receptor) alterations that are expressed by the VISA strains investigated. Collectively, these findings indicate that inappropriate relationships between VISA and vancomycin-susceptible parents might be drawn if only phage-typing and antibiotic susceptibility are utilized to determine epidemiological relationships.

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2003-08-01
2024-03-29
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