1887

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Volume 62, Issue 9

Molecular genotyping of spp. isolated in Arizona, USA, using multilocus PCR and mass spectrometry Free

Abstract

spp. are a diverse group of Gram-negative bacteria frequently implicated in nosocomial infections. Genotypic methods have been instrumental in studying , but few offer high resolution, rapid turnaround time, technical ease and high inter-laboratory reproducibility, which has hampered understanding of disease incidence, transmission patterns and diversity within this genus. Here, we further evaluated multilocus PCR electrospray ionization/mass spectrometry (PCR/ESI-MS), a method that is simple and robust, and provides both species characterization and strain-level resolution of spp. on a single platform. We examined 125 isolates from 21 hospitals, laboratories and medical centres spanning four counties in Arizona, USA, using PCR/ESI-MS. We compared PCR/ESI-MS with an in-house amplified fragment length polymorphism (AFLP) genotyping scheme. PCR/ESI-MS demonstrated that spp. from Arizonan hospitals had similar species and strain distributions to other US civilian hospitals. Furthermore, we showed that the PCR/ESI-MS and AFLP genotypes were highly congruent, with the former having the advantages of robust inter-laboratory reproducibility, rapid turnaround time and simple experimental set-up and data analysis. PCR/ESI-MS is an effective and high-throughput platform for strain typing of and for identification of other spp., including the emerging nosocomial pathogens and .

  • Received:
  • Accepted:
  • Published Online:
© 2013 SGM
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2013-09-01
2024-04-18
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Molecular genotyping of Acinetobacter spp. isolated in Arizona, USA, using multilocus PCR and mass spectrometry
J. Med. Microbiol. 62, 1295 (2013); https://doi.org/10.1099/jmm.0.052381-0
/content/journal/jmm/10.1099/jmm.0.052381-0
/content/journal/jmm/10.1099/jmm.0.052381-0
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