Multilocus sequence typing of DNA from faecal specimens for the analysis of intra-familial transmission of Helicobacter pylori Osaki, Takako and Okuda, Masumi and Ueda, Junko and Konno, Mutsuko and Yonezawa, Hideo and Hojo, Fuhito and Yagyu, Kiyoko and Lin, Yingsong and Fukuda, Yoshihiro and Kikuchi, Shogo and Kamiya, Shigeru,, 62, 761-765 (2013), doi = https://doi.org/10.1099/jmm.0.053140-0, publicationName = Microbiology Society, issn = 0022-2615, abstract= This study used multilocus sequence typing (MLST) of total DNA extracted from faecal specimens to genotype Helicobacter pylori to analyse intra-familial transmission. Faecal DNA was extracted and amplified by nested PCR. The products were analysed by direct sequencing and the allele type was determined using an MLST website. Mother-to-child transmission was suspected in at least two of three families, and father-to-child transmission was suspected in one family., language=, type=