RT Journal Article SR Electronic(1) A1 Osaki, Takako A1 Okuda, Masumi A1 Ueda, Junko A1 Konno, Mutsuko A1 Yonezawa, Hideo A1 Hojo, Fuhito A1 Yagyu, Kiyoko A1 Lin, Yingsong A1 Fukuda, Yoshihiro A1 Kikuchi, Shogo A1 Kamiya, ShigeruYR 2013 T1 Multilocus sequence typing of DNA from faecal specimens for the analysis of intra-familial transmission of Helicobacter pylori JF Journal of Medical Microbiology, VO 62 IS 5 SP 761 OP 765 DO https://doi.org/10.1099/jmm.0.053140-0 PB Microbiology Society, SN 1473-5644, AB This study used multilocus sequence typing (MLST) of total DNA extracted from faecal specimens to genotype Helicobacter pylori to analyse intra-familial transmission. Faecal DNA was extracted and amplified by nested PCR. The products were analysed by direct sequencing and the allele type was determined using an MLST website. Mother-to-child transmission was suspected in at least two of three families, and father-to-child transmission was suspected in one family., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.053140-0