1887

Abstract

Vi polysaccharide and iron-regulated outer-membrane proteins (IROMPs) were extracted and purified from the standard strain of , Ty2. These were then conjugated by chemical coupling using the carbodimide method. Vi–IROMP conjugate was tested for its ability to protect against colonization by in different organs. Mice immunized with 2.5 μg Vi–IROMP conjugate showed the most protection, as the least bacterial colonization of spleen, liver and Peyer's patches was observed. Peritoneal macrophages obtained from conjugate-treated mice phagocytosed bacteria efficiently. Circulating antibodies and the delayed type hypersensitivity response elucidated by mouse foot-pad swelling was significantly higher in conjugate-treated animals. These results clearly demonstrate that an IROMP and polysaccharide conjugate of prepared from the same strain has the potential to protect animals against challenge.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.05378-0
2004-07-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jmm/53/7/JM530718.html?itemId=/content/journal/jmm/10.1099/jmm.0.05378-0&mimeType=html&fmt=ahah

References

  1. Acharya I. L., Lowe C. U., Thapa R., Gurubacharya V. L., Shrestha M. B., Cadoz M., Schulz D., Armand J., Bryla D. A., Trollfors B. other authors 1987; Prevention of typhoid fever in Nepal with Vi capsular polysaccharide of Salmonella typhi .A preliminary report. N Engl J Med 317:1101–1104 [CrossRef]
    [Google Scholar]
  2. Allen P. M., Fisher D., Saunders J. R., Hart C. A. 1987; The role of capsular polysaccharide K21b of Klebsiella and of the structurally related colanic-acid polysaccharide of Escherichia coli in resistance to phagocytosis and serum killing. J Med Microbiol 24:363–370 [CrossRef]
    [Google Scholar]
  3. Banerjee-Bhatnagar N., Frasch C. E. 1990; Expression of Neisseria meningitidis iron-regulated outer membrane proteins including a 70-kilodalton transferrin receptor, and their potential for use as vaccines. Infect Immun 58:2875–2881
    [Google Scholar]
  4. Beuvery E. C., van Rossum F., Nagel J. 1982; Comparison of the induction of immunoglobulins M and G antibodies in mice with purified pneumococcal type 3 and meningococcal group C polysaccharides and their protein conjugates. Infect Immun 37:15–22
    [Google Scholar]
  5. Cadoz M. 1998; Potential and limitations of polysaccharide vaccines in infancy. Vaccine 16:1391–1395 [CrossRef]
    [Google Scholar]
  6. Chibber S., Bajaj J. 1995; Polysaccharide-iron-regulated cell surface protein conjugate vaccine its role in protection against Klebsiella pneumoniae -induced lobar pneumonia. Vaccine 13:179–184 [CrossRef]
    [Google Scholar]
  7. Desiderio J. V., Campbell S. G. 1985; Immunization against experimental murine salmonellosis with liposome-associated O-antigen. Infect Immun 48:658–663
    [Google Scholar]
  8. Filip C., Fletcher G., Wulff J. L., Earhart C. F. 1973; Solubilization of cytoplasmic membrane of Escherichia coli by ionic detergent sodium-lauryl sarcosinate. J Bacteriol 115:717–772
    [Google Scholar]
  9. Frantz I. D. 1942; Growth requirements of the meningococcus. J Bacteriol 43:757–761
    [Google Scholar]
  10. Gotschlich E. C. 1975; Development of polysaccharide vaccines for the prevention of meningococcal diseases. Monogr Allergy 9:245–258
    [Google Scholar]
  11. Guerinot M. L. 1994; Microbial iron transport. Annu Rev Microbiol 48:743–772 [CrossRef]
    [Google Scholar]
  12. Kossaczka Z., Lin F. Y., Ho V. A. & 11 other authors; 1999; Safety and immunogenicity of Vi conjugate vaccines for typhoid fever in adults, teenagers, and 2- to 4-year old children in Vietnam. Infect Immun 67:5806–5810
    [Google Scholar]
  13. Kussi N., Nurminen M., Saxen H., Valtonen M., Makela P. H. 1979; Immunization with major outer membrane proteins in experimental salmonellosis of mice. Infect Immun 25:857–862
    [Google Scholar]
  14. Laemmli U. K. 1970; Cleavage of structural properties during the assembly of the head of bacteriophage T4. Nature 227:680–685 [CrossRef]
    [Google Scholar]
  15. Lin F. Y., Ho V. A., Khiem H. B. & 9 other authors; 2001; The efficacy of a Salmonella typhi Vi conjugate vaccine in two-to-five-year-old children. N Engl J Med 344:1263–1269 [CrossRef]
    [Google Scholar]
  16. Lucier T. S., Fetherston J. D., Brubaker R. R., Perry R. D. 1996; Iron uptake and iron-repressible polypeptides in Yersinia pestis . Infect Immun 64:3023–3031
    [Google Scholar]
  17. Paniagua J., Garcia J. A., Lopez C. R., Gonzalez C. R., Isibasi A., Kumate J. 1992; Conjugate vaccines against bacterial infections: typhoid fever. Salud Publica Mex 34:268–273 (in Spanish
    [Google Scholar]
  18. Reissbrodt R., Kingsley R., Rabsch W., Beer W., Roberts M., Williams P. H. 1997; Iron regulated excretion of acids by S.typhimurium . J Bacteriol 179:4538–4544
    [Google Scholar]
  19. Robbins J. B., Schneerson R. 1990; Polysaccharide-protein conjugates: a new generation of vaccines. J Infect Dis 161:821–832 [CrossRef]
    [Google Scholar]
  20. Singh M., Ganguly N. K., Kumar L., Vohra H. 1999; Protective efficacy and immunogenicity of Vi-porin conjugate against Salmonella typhi . Microbiol Immunol 43:535–542 [CrossRef]
    [Google Scholar]
  21. Szu S. C., Taylor D. N., Trofa A. C., Clements J. D., Shiloach J., Sadoff J. C., Bryla D. A., Robbins J. B. 1994; Laboratory and preliminary clinical characterization of Vi capsular polysaccharide-protein conjugate vaccines. Infect Immun 62:4440–4444
    [Google Scholar]
  22. Williams P., Lambert P. A., Brown M. K. W., James R. J. 1983; Role of the O and R antigen in determining antimicrobial agents. Chemotherapy 31:7562–7585
    [Google Scholar]
  23. Yang H. H., Wu C. G., Xie G. Z. & 17 other authors; 2001; Efficacy trial of Vi polysaccharide vaccine against typhoid fever in south-western China. Bull World Health Organ 79:625–631
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.05378-0
Loading
/content/journal/jmm/10.1099/jmm.0.05378-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error