1887

Abstract

In a recent study, a large proportion of multi-drug-resistant (MDR) strains that were isolated from hospitalized patients in the Czech Republic was found to belong to two major groups (A and B). These groups appeared to be similar to epidemic clones I and II, respectively, which were identified previously among outbreak strains from north-western European hospitals. The aim of the present study was to assess in detail the genetic relatedness of Czech strains and those of epidemic clones I and II by using ribotyping with dIII and cII and by AFLP fingerprinting. The study collection included 70 MDR strains that were isolated in 30 Czech hospitals in 1991–2001, 15 susceptible Czech strains from 1991 to 1996 and 13 reference strains of clones I and II from 1982 to 1990. One major dIII/cIII ribotype (-) was observed in 38 MDR Czech strains and eight reference strains of clone I, whereas another major ribotype (-) was observed in 11 MDR Czech strains and in three reference strains of clone II. A selection of 59 Czech strains (representative of all ribotypes) and the 13 reference strains were investigated by AFLP fingerprinting. At a clustering level of 83 %, two large clusters could be distinguished: cluster 1 included all reference strains of clone I and 25 MDR Czech strains, whilst cluster 2 contained all reference strains of clone II and 11 MDR Czech strains. There was a clear correlation between the groupings by AFLP analysis and by ribotyping, as all strains with ribotype - and four strains with slightly different ribotypes were found in AFLP cluster 1, whereas all strains with ribotype - and seven strains with similar ribotypes were in AFLP cluster 2. Thus, 41 and 21 MDR Czech strains could be classified as belonging to clones I and II, respectively. The remaining eight MDR and 15 susceptible strains were highly heterogeneous and were distinct from clones I and II by both AFLP fingerprinting and ribotyping. These results indicate that the two predominant groups observed among MDR Czech strains from the 1990s are genetically congruent with the north-western European epidemic clones that were found in the 1980s. Recognition of these clinically relevant, widespread clones is important in infection prevention and control; they are also interesting subjects to study genetic mechanisms that give rise to their antibiotic resistance and epidemic behaviour.

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2004-02-01
2024-03-28
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References

  1. Aygun G., Demirkiran O., Utku T., Mete B., Urkmez S., Yilmaz M., Yasar H., Dikmen Y., Ozturk R. 2002; Environmental contamination during a carbapenem-resistant Acinetobacter baumannii outbreak in an intensive care unit. J Hosp Infect 52:259–262 [CrossRef]
    [Google Scholar]
  2. Bergogne-Bérézin E., Towner K. J. 1996; Acinetobacter spp.as nosocomial pathogens: microbiological, clinical, and epidemiological features. Clin Microbiol Rev 9:148–165
    [Google Scholar]
  3. Bouvet P. J. M., Grimont P. A. D. 1987; Identification and biotyping of clinical isolates of Acinetobacter . Ann Inst Pasteur Microbiol 138:569–578 [CrossRef]
    [Google Scholar]
  4. Brisse S., Milatovic D., Fluit A. C., Kusters K., Toelstra A., Verhoef J., Schmitz F.-J. 2000; Molecular surveillance of European quinolone-resistant clinical isolates of Pseudomonas aeruginosa and Acinetobacter spp.using automated ribotyping. J Clin Microbiol 38:3636–3645
    [Google Scholar]
  5. Carr E. L., Kämpfer P., Patel B. K. C., Gürtler V., Seviour R. J. 2003; Seven novel species of Acinetobacter isolated from activated sludge. Int J Syst Evol Microbiol 53:953–963 [CrossRef]
    [Google Scholar]
  6. Dijkshoorn L., Aucken H., Gerner-Smidt P., Janssen P., Kaufmann M. E., Garaizar J., Ursing J., Pitt T. L. 1996; Comparison of outbreak and nonoutbreak Acinetobacter baumannii strains by genotypic and phenotypic methods. J Clin Microbiol 34:1519–1525
    [Google Scholar]
  7. Henriksen S. D. 1973; Moraxella , Acinetobacter , and the Mimeae . Bacteriol Rev 37:522–561
    [Google Scholar]
  8. Janssen P., Dijkshoorn L. 1996; High resolution DNA fingerprinting of Acinetobacter outbreak strains. FEMS Microbiol Lett 142:191–194 [CrossRef]
    [Google Scholar]
  9. Juni E. 1984; Genus III. Acinetobacter Brisou and Prévot 1954, 727AL . In Bergey's Manual of Systematic Bacteriology vol. 1 pp 303–307 Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  10. Lautrop H. 1974; Acinetobacter . In Bergey's Manual of Determinative Bacteriology . pp 436–438 Edited by Buchanan R. E., Gibbons N. E. Baltimore: Williams & Wilkins;
  11. NCCLS 2001 Performance Standards for Antimicrobial Susceptibility Testing: 11th informational supplement (document M100-S11 Wayne, PA: NCCLS;
    [Google Scholar]
  12. Nemec A. 1999; Use of the disc diffusion test for epidemiological typing of multiresistant Acinetobacter baumannii strains. Klin Mikrobiol Infect Lék 5:287–297 (in Czech
    [Google Scholar]
  13. Nemec A., Janda L., Melter O., Dijkshoorn L. 1999; Genotypic and phenotypic similarity of multiresistant Acinetobacter baumannii isolates in the Czech Republic. J Med Microbiol 48:287–296 [CrossRef]
    [Google Scholar]
  14. Nemec A., De Baere T., Tjernberg I., Vaneechoutte M., van der Reijden T. J. K., Dijkshoorn L. 2001; Acinetobacter ursingii sp.nov. and Acinetobacter schindleri sp. nov., isolated from human clinical specimens. Int J Syst Evol Microbiol 51:1891–1899 [CrossRef]
    [Google Scholar]
  15. Nemec A., Dijkshoorn L., Cleenwerck I., De Baere T., Janssens D., van der Reijden T. J. K., Ježek P., Vaneechoutte M. 2003; Acinetobacter parvus sp.nov., a small-colony-forming species isolated from human clinical specimens. Int J Syst Evol Microbiol 53:1563–1567 [CrossRef]
    [Google Scholar]
  16. Pantophlet R., Nemec A., Brade L., Brade H., Dijkshoorn L. 2001; O-antigen diversity among Acinetobacter baumannii strains from the Czech Republic and northwestern Europe, as determined by lipopolysaccharide-specific monoclonal antibodies. J Clin Microbiol 39:2576–2580 [CrossRef]
    [Google Scholar]
  17. Pantophlet R., Severin J. A., Nemec A., Brade L., Dijkshoorn L., Brade H. 2002; Identification of Acinetobacter isolates from species belonging to the Acinetobacter calcoaceticus-Acinetobacter baumannii complex with monoclonal antibodies specific for O antigens of their lipopolysaccharides. Clin Diagn Lab Immunol 9:60–65
    [Google Scholar]
  18. Seifert H., Gerner-Smidt P. 1995; Comparison of ribotyping and pulsed-field gel electrophoresis for molecular typing of Acinetobacter isolates. J Clin Microbiol 33:1402–1407
    [Google Scholar]
  19. Seifert H., Baginski R., Schulze A., Pulverer G. 1993; The distribution of Acinetobacter species in clinical culture materials. Zentbl Bakteriol 279:544–552 [CrossRef]
    [Google Scholar]
  20. van Dessel H., Dijkshoorn L., van der Reijden T., Bakker N., Paauw A., van den Broek P., Verhoef J., Brisse S. 2003; Identification of a new geographically widespread multiresistant Acinetobacter baumannii clone from European hospitals. Res Microbiol (in press http://dx.doi.org/10.1016/j.resmic.2003.10.003
    [Google Scholar]
  21. Wang S. H., Sheng W. H., Chang Y. Y. & 7 other authors; 2003; Healthcare-associated outbreak due to pan-drug resistant Acinetobacter baumannii in a surgical intensive care unit. J Hosp Infect 53:97–102 [CrossRef]
    [Google Scholar]
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