1887

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Volume 62, Issue 8

High prevalence of VIM-4 and NDM-1 metallo-β-lactamase among carbapenem-resistant Free

Abstract

The purpose of this study was to identify the mechanisms leading to carbapenem resistance among multidrug-resistant isolates recovered from hospitalized patients with nosocomial infections in Mubarak Al Kabeer Hospital, Kuwait. Fourteen carbapenem-resistant isolates were obtained from inpatients in different wards and intensive care units between April 2009 and February 2011. Antibiotic susceptibilities were determined using the E-test method. Genes encoding β-lactamases were characterized by specific PCR amplification, sequencing and conjugation assays. All isolates were identified as metallo-β-lactamase (MBL) producers using phenotypic and molecular methods. Eleven of the 14 isolates produced VIM-4 (six , three , one and one ). Three isolates produced the MBL NDM-1 and co-produced the plasmid-encoded AmpC CMY-4. The VIM-4-producing isolates co-produced extended-spectrum β-lactamases including CTX-M-15 and some SHV derivatives. The VIM-4 gene was not transferable by conjugation studies of six selected strains. We demonstrated here the emergence of VIM-4- and NDM-1-producing isolates in the largest teaching hospital in Kuwait.

  • Received:
  • Accepted:
  • Published Online:
© 2013 SGM
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2013-08-01
2024-04-23
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High prevalence of VIM-4 and NDM-1 metallo-β-lactamase among carbapenem-resistant Enterobacteriaceae
J. Med. Microbiol. 62, 1239 (2013); https://doi.org/10.1099/jmm.0.059915-0
/content/journal/jmm/10.1099/jmm.0.059915-0
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