1887

Abstract

is a species of the order recently described and reported as a cause of human mucormycosis. We report a case of in a man involved in a serious sailing accident causing deep skin and soft tissue contamination with soil and water. Direct microscopic examination of the clinical sample with Giemsa stains showed hyaline and non-septate hyphae belonging to the order . Fungal identification was performed by culture of biopsy material on SDA, and identification of species by floating an agar block containing the fungus in a nutritionally deficient medium consisting of sterile distilled water supplemented with 0.05 % yeast extract; and by sequencing the ITS region of the rDNA. This is the first report to our knowledge of infection with in Argentina, confirming the presence of this fungus in this country.

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2014-02-01
2024-04-25
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References

  1. Alvarez E., Garcia-Hermoso D., Sutton D. A., Cano J. F., Stchigel A. M., Hoinard D., Fothergill A. W., Rinaldi M. G., Dromer F., Guarro J. 2010; Molecular phylogeny and proposal of two new species of the emerging pathogenic fungus Saksenaea. . J Clin Microbiol 48:4410–4416 [View Article][PubMed]
    [Google Scholar]
  2. Balajee S. A., Borman A. M., Brandt M. E., Cano J., Cuenca-Estrella M., Dannaoui E., Guarro J., Haase G., Kibbler C. C. other authors 2009; Sequence-based identification of Aspergillus, Fusarium, and Mucorales species in the clinical mycology laboratory: where are we and where should we go from here?. J Clin Microbiol 47:877–884 [View Article][PubMed]
    [Google Scholar]
  3. Baradkar V. P., Kumar S. 2009; Cutaneous zygomycosis due to Saksenaea vasiformis in an immunocompetent host. Indian J Dermatol 54:382–384 [View Article][PubMed]
    [Google Scholar]
  4. Bearer E. A., Nelson P. R., Chowers M. Y., Davis C. E. 1994; Cutaneous zygomycosis caused by Saksenaea vasiformis in a diabetic patient. J Clin Microbiol 32:1823–1824[PubMed]
    [Google Scholar]
  5. Blanchet D., Dannaoui E., Fior A., Huber F., Couppié P., Salhab N., Hoinard D., Aznar C. 2008; Saksenaea vasiformis infection, French Guiana. Emerg Infect Dis 14:342–344 [View Article][PubMed]
    [Google Scholar]
  6. Chakrabarti A., Kumar P., Padhye A. A., Chatha L., Singh S. K., Das A., Wig J. D., Kataria R. N. 1997; Primary cutaneous zygomycosis due to Saksenaea vasiformis and Apophysomyces elegans. . Clin Infect Dis 24:580–582 [View Article][PubMed]
    [Google Scholar]
  7. García-Martínez J., López-Medrano F., Alhambra A., del Palacio A. 2008; Rhinocerebral zygomycosis caused by Saksenae vasiformis in a diabetic patient. Mycoses 51:549–553 [View Article][PubMed]
    [Google Scholar]
  8. Gomes M. Z. R., Lewis R. E., Kontoyiannis D. P. 2011; Mucormycosis caused by unusual mucormycetes, non-Rhizopus, -Mucor, and -Lichtheimia species. Clin Microbiol Rev 24:411–445 [View Article][PubMed]
    [Google Scholar]
  9. Guarro J. 2012; [Taxonomy and biology of fungi causing human infection.] . Enferm Infecc Microbiol Clin 30:33–39 (in Spanish) [View Article][PubMed]
    [Google Scholar]
  10. Hospenthal D. R., Chung K. K., Lairet K., Thompson E. H., Guarro J., Renz E. M., Sutton D. A. 2011; Saksenaea erythrospora infection following combat trauma. J Clin Microbiol 49:3707–3709 [View Article][PubMed]
    [Google Scholar]
  11. Iwen P. C., Hinrichs S. H., Rupp M. E. 2002; Utilization of the internal transcribed spacer regions as molecular targets to detect and identify human fungal pathogens. Med Mycol 40:87–109[PubMed] [CrossRef]
    [Google Scholar]
  12. Kontoyiannis D. P., Lewis R. E. 2011; How I treat mucormycosis. Blood 118:1216–1224 [View Article][PubMed]
    [Google Scholar]
  13. Lawhon S. D., Corapi W. V., Hoffmann A. R., Libal M. C., Alvarez E., Guarro J., Wickes B. L., Fu J., Thompson E. H., Sutton D. A. 2012; In utero infection of a calf by Saksenaea erythrospora resulting in neonatal abomasitis and dermatitis. J Vet Diagn Invest 24:990–993 [View Article][PubMed]
    [Google Scholar]
  14. Lechevalier P., Hermoso D. G., Carol A., Bonacorsi S., Ferkdadji L., Fitoussi F., Lortholary O., Bourrillon A., Faye A. other authors 2008; Molecular diagnosis of Saksenaea vasiformis cutaneous infection after scorpion sting in an immunocompetent adolescent. J Clin Microbiol 46:3169–3172 [View Article][PubMed]
    [Google Scholar]
  15. Mayayo E., Stchigel A. M., Cano J. F., Bernal-Escoté X., Guarro J. 2013; [Necrotizing fasciitis caused by Saksenaea vasiformis in an immunocompetent patient after a car accident.]. Rev Iberoam Micol 30:57–60 (in Spanish) [View Article][PubMed]
    [Google Scholar]
  16. Oberle A. D., Penn R. L. 1983; Nosocomial invasive Saksenaea vasiformis infection. Am J Clin Pathol 80:885–888[PubMed]
    [Google Scholar]
  17. Padhye A. A., Ajello L. 1988; Simple method of inducing sporulation by Apophysomyces elegans and Saksenaea vasiformis. . J Clin Microbiol 26:1861–1863[PubMed]
    [Google Scholar]
  18. Parker C., Kaminski G., Hill D. 1986; Zygomycosis in a tattoo, caused by Saksenaea vasiformis. . Australasian J Dermatol 27:107–111 [View Article][PubMed]
    [Google Scholar]
  19. Rogers T. R. 2008; Treatment of zygomycosis: current and new options. J Antimicrob Chemother 61:Suppl. 1i35–i40 [View Article][PubMed]
    [Google Scholar]
  20. Spellberg B., Edwards J. Jr, Ibrahim A. 2005; Novel perspectives on mucormycosis: pathophysiology, presentation, and management. Clin Microbiol Rev 18:556–569 [View Article][PubMed]
    [Google Scholar]
  21. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011; MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739 [View Article][PubMed]
    [Google Scholar]
  22. Vega W., Orellana M., Zaror L., Gené J., Guarro J. 2006; Saksenaea vasiformis infections: case report and literature review. Mycopathologia 162:289–294 [View Article][PubMed]
    [Google Scholar]
  23. Walther G., Pawłowska J., Alastruey-Izquierdo A., Wrzosek M., Rodriguez-Tudela J. L., Dolatabadi S., Chakrabarti A., de Hoog G. S. 2013; DNA barcoding in Mucorales: an inventory of biodiversity. Persoonia 30:11–47 [View Article][PubMed]
    [Google Scholar]
  24. White T. J., Bruns T., Lee S., Taylor J. 1990; Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols, a Guide to Methods and Applications pp. 315–322 Edited by Innis M. A., Gelfand D. H., Sninsky J. J., White T. J. San Diego, CA: Academic Press;
    [Google Scholar]
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