Evaluation of a commercial real-time PCR for the detection of extended spectrum β-lactamase genes Willemsen, Ina and Hille, Linda and Vrolijk, Angela and Bergmans, Anneke and Kluytmans, Jan,, 63, 540-543 (2014), doi = https://doi.org/10.1099/jmm.0.070110-0, publicationName = Microbiology Society, issn = 0022-2615, abstract= We investigated the performance of a real-time PCR for the detection of extended spectrum β-lactamase genes in Enterobacteriaceae (Check-MDR ESBL PCR). Results from micro-arrays were considered as the gold standard. An analysis on 489 isolates resulted in a sensitivity of 98.9 % and a specificity of 100 % for the PCR., language=, type=