1887

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Volume 57, Issue 12

Antibody response to the 45 kDa antigen in an animal model and potential role of the antigen in adherence Free

Abstract

The antigen CR3-RP (complement receptor 3-related protein) is supposed to be a ‘mimicry’ protein because of its ability to bind antibody directed against the subunit of the mammalian CR3 (CD11b/CD18). This study aimed to (i) investigate the specific humoral isotypic response to immunization with CR3-RP in a rabbit animal model, and (ii) determine the role of CR3-RP in the adherence of using the model systems of buccal epithelial cells (BECs) and biofilm formation. The synthetic peptide DINGGGATLPQ corresponding to 11 amino-acids of the CR3-RP sequence DINGGGATLPQALXQITGVIT, determined by N-terminal sequencing, was used for immunization of rabbits to obtain polyclonal anti-CR3-PR serum and for subsequent characterization of the humoral isotypic response of rabbits. A significant increase of IgG, IgA and IgM anti-CR3-RP specific antibodies was observed after the third (<0.01) and the fourth (<0.001) immunization doses. The elevation of IgA levels suggested peptide immunomodulation of the IgA1 subclass, presumably in coincidence with epithelial adherence. Blocking CR3-RP with polyclonal anti-CR3-RP serum reduced the ability of to adhere to BECs, in comparison with the control, by up to 35 % (<0.001), and reduced biofilm formation by 28 % (<0.001), including changes in biofilm thickness and integrity detected by confocal laser scanning microscopy. These properties of CR3-RP suggest that it has potential for future vaccine development.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): BEC, buccal epithelial cell , CLSM, confocal laser scanning microscopy , RT, room temperature and XTT, 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide
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2008-12-01
2024-04-27
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Antibody response to the 45 kDa Candida albicans antigen in an animal model and potential role of the antigen in adherence
J. Med. Microbiol. 57, 1466 (2008); https://doi.org/10.1099/jmm.0.2008/001479-0
/content/journal/jmm/10.1099/jmm.0.2008/001479-0
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