1887

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Volume 57, Issue 11

Identification and molecular analysis of C–S lyase producing hydrogen sulfide in Free

Abstract

Hydrogen sulfide (HS) is a toxic gas that induces the modification and release of haemoglobin in erythrocytes; however, it also functions in methionine biosynthesis in bacteria. C–S lyase, encoded by the gene, is responsible for bacterial HS production through the cleavage of -cysteine. In this study, 26 of 29 crude extracts from reference and clinical strains of produced HS from -cysteine. The capacities in those strains were not higher than those in strains of the other anginosus group of streptococci, and , but were much greater than those in strains of , which is known to have an extremely low capacity for HS production. Incubation of the remaining three extracts with -cysteine did not result in HS production. Sequence analysis revealed that the genes from these three strains ( strains ATCC 27335, IMU151 and IMU202) contained mutations or small deletions. HS production in crude extracts prepared from ATCC 27335 was restored by repairing the gene sequence in genomic DNA. The kinetic properties of the purified recombinant protein encoded by the repaired gene were comparable to those of native proteins produced by HS-producing strains, whereas the truncated protein produced by ATCC 27335 had no enzymic activity with -cysteine or -cystathionine. However, real-time PCR analysis indicated that the gene in strains ATCC 27335, IMU151 and IMU202 is transcribed and regulated in a manner similar to that in the HS-producing strain.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): GST, glutathione S-transferase and PLP, pyridoxal 5′-phosphate
SGM
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2008-11-01
2024-04-25
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Identification and molecular analysis of βC–S lyase producing hydrogen sulfide in Streptococcus intermedius
J. Med. Microbiol. 57, 1411 (2008); https://doi.org/10.1099/jmm.0.2008/001677-0
/content/journal/jmm/10.1099/jmm.0.2008/001677-0
/content/journal/jmm/10.1099/jmm.0.2008/001677-0
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