Coccidioidal pericarditis: a rapid presumptive diagnosis by an in-house antigen confirmed by mycological and molecular methods
Brilhante, Raimunda S. N. and Cordeiro, Rossana A. and Rocha, Marcos F. G. and Fechine, Maria A. B. and Furtado, Felipe M. and Nagao-Dias, Aparecida T. and Camargo, Zoilo P. and Sidrim, José J. C.,, 57, 1288-1292 (2008),
doi = https://doi.org/10.1099/jmm.0.2008/002428-0,
publicationName = Microbiology Society,
issn = 0022-2615,
abstract= Coccidioidal pericarditis is a condition found in approximately 1–5 % of patients infected by Coccidioides species. It is associated with widely diverse clinical symptoms. This paper reports a case of coccidioidal pericarditis diagnosed by an in-house Coccidioides posadasii antigen and confirmed with mycological and molecular methods. From February to September 2005, the patient suffered from fever, weight loss, a non-productive cough, thoracic pain and tachycardia. He received a positive diagnosis of coccidioidal pericarditis only in October 2005. The macromorphological examination of the culture showed a whitish felt-like colony, which became brownish with age. Preparations in lactophenol cotton blue stain showed hyaline septate hyphae with fragmentation and thin arthroconidia-like structures. Pericardial fluid and sera samples were positive for Coccidioides antibodies by immunodiffusion and ELISA with a C. posadasii in-house antigen preparation. The C. posadasii identification was confirmed by nested PCR of the antigen 2/proline-rich antigen (Ag2/PRA) encoding gene.,
language=,
type=