RT Journal Article SR Electronic(1) A1 Bai, Zhijun A1 Liu, Licheng A1 Tu, Zeng A1 Yao, Lisi A1 Liu, Jianwei A1 Xu, Bing A1 Tang, Boheng A1 Liu, Jinhua A1 Wan, Yongji A1 Fang, Meiyu A1 Chen, WeijunYR 2008 T1 Real-time PCR for detecting circulating dengue virus in the Guangdong Province of China in 2006 JF Journal of Medical Microbiology, VO 57 IS 12 SP 1547 OP 1552 DO https://doi.org/10.1099/jmm.0.2008/003418-0 PB Microbiology Society, SN 1473-5644, AB Dengue virus (DENV) causes a wide range of diseases in humans, from the acute febrile illness dengue fever (DF) to life-threatening dengue haemorrhagic fever/dengue shock syndrome. We developed four real-time quantitative PCR assays for each serotype of DENV based on computational analysis. These assays had high sensitivity and specificity without cross-reactivity for the four serotypes. To evaluate the performance of these assays in detecting and typing the virus in clinical samples, we analysed 64 serum samples from Guangdong during 2006. The results showed that 71 % of those samples were positive by the DEN-1 assay. The DENV assay results, in agreement with the serological tests and sequencing analysis, showed that the pathogen resulting in the DF explosion in Guangdong in 2006 belonged to DEN-1. Compared to the serological assays, the real-time PCR assays that we developed were much more sensitive in the 1–3 days after onset of the symptoms., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.2008/003418-0