1887

Abstract

The genetic characteristics of strains isolated in 2004 and 2005 in Mozambique were assessed in this study to determine whether the pandemic clone of O3 : K6 and O4 : K68 serotypes has spread to Mozambique. Fifty-eight strains isolated from hospitalized diarrhoea patients in Beira, Mozambique, were serotyped for O : K antigens and genotyped for , and genes. A group-specific PCR, a PCR that detects the presence of ORF8 of the filamentous phage f237, arbitrarily primed PCR, PFGE and multilocus sequence typing were performed to determine the pandemic status of the strains and their ancestry. All strains of serovars O3 : K6 (=38) and O4 : K68 (=4) were identified as a pandemic clonal group by these analyses. These strains are closely related to the pandemic reference strains of O3 : K6 and O4 : K68, which emerged in Asia in 1996 and were later found globally. The pandemic serotypes O3 : K6 and O4 : K68 including reference strains grouped into a single cluster indicating emergence from a common ancestor. The O3 : K58 (=8), O4 : K13 (=6), O3 : KUT (=1) and O8 : K41 (=1) strains showed unique characteristics different from the pandemic clone.

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2008-12-01
2024-03-28
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References

  1. Ansaruzzaman M., Lucas M., Deen J. L., Bhuiyan N. A., Wang X. Y., Safa A., Sultana M., Chowdhury A., Nair G. B. other authors 2005; Pandemic serovars (O3 : K6 and O4 : K68) of Vibrio parahaemolyticus associated with diarrhea in Mozambique: spread of the pandemic into the African continent. J Clin Microbiol 43:2559–2562 [CrossRef]
    [Google Scholar]
  2. Bhuiyan N. A., Ansaruzzaman M., Kamruzzaman M., Alam K., Chowdhury N. R., Nishibuchi M., Faruque S. M., Sack D. A., Takeda Y., Nair G. B. 2002; Prevalence of the pandemic genotype of Vibrio parahaemolyticus in Dhaka, Bangladesh, and significance of its distribution across different serotypes. J Clin Microbiol 40:284–286 [CrossRef]
    [Google Scholar]
  3. Chowdhury N. R., Chakraborty S., Ramamurthy T., Nishibuchi M., Yamasaki S., Takeda Y., Nair G. B. 2000; Molecular evidence of clonal Vibrio parahaemolyticus pandemic strains. Emerg Infect Dis 6:631–636 [CrossRef]
    [Google Scholar]
  4. Chowdhury A., Ishibashi M., Thiem V. D., Tuyet D. T., Tung T. V., Chien B. T., von Seidlein L., Canh D. G., Clemens J. other authors 2004; Emergence and serovar transition of Vibrio parahaemolyticus pandemic strains isolated during a diarrhea outbreak in Vietnam between 1997 and 1999. Microbiol Immunol 48:319–327 [CrossRef]
    [Google Scholar]
  5. Cooper K. L., Luey C. K., Bird M., Terajima J., Nair G. B., Kam K. M., Arakawa E., Safa A., Cheung D. T. other authors 2006; Development and validation of a PulseNet standardized pulsed-field gel electrophoresis protocol for subtyping of Vibrio cholerae . Foodborne Pathog Dis 3:51–58 [CrossRef]
    [Google Scholar]
  6. Gil A. I., Miranda H., Lanata C. F., Prada A., Hall E. R., Barreno C. M., Nusrin S., Bhuiyan N. A., Sack D. A., Nair G. B. (2007).O3 : K6 serotype of Vibrio parahaemolyticus identical to the global pandemic clone associated with diarrhea in Peru. Int J Infect Dis 11:324–328 [CrossRef]
    [Google Scholar]
  7. González-Escalona N., Martinez-Urtaza J., Romero J., Espejo R. T., Jaykus L. A., DePaola A. 2008; Determination of molecular phylogenetics of Vibrio parahaemolyticus strains by multilocus sequence typing. J Bacteriol 190:2831–2840 [CrossRef]
    [Google Scholar]
  8. Hunter S. B., Vauterin P., Lambert-Fair M. A., Van Duyne M. S., Kubota K., Graves L., Wrigley D., Barrett T., Ribot E. 2005; Establishment of a universal size standard strain for use with the PulseNet standardized pulsed-field gel electrophoresis protocols: converting the national databases to the new size standard. J Clin Microbiol 43:1045–1050 [CrossRef]
    [Google Scholar]
  9. Matsumoto C., Okuda J., Ishibashi M., Iwanaga M., Garg P., Rammamurthy T., Wong H. C., Depaola A., Kim Y. B. other authors 2000; Pandemic spread of an O3 : K6 clone of Vibrio parahaemolyticus and emergence of related strains evidenced by arbitrarily primed PCR and toxRS sequence analyses. J Clin Microbiol 38:578–585
    [Google Scholar]
  10. Nair G. B., Ramamurthy T., Bhattacharya S. K., Dutta B., Takeda Y., Sack D. A. 2007; Global dissemination of Vibrio parahaemolyticus serotype O3 : K6 and its serovariants. Clin Microbiol Rev 20:39–48 [CrossRef]
    [Google Scholar]
  11. Nasu H., Iida T., Sugahara T., Yamaichi Y., Park K. S., Yokoyama K., Makino K., Shinagawa H., Honda T. 2000; A filamentous phage associated with recent pandemic Vibrio parahaemolyticus O3 : K6 strains. J Clin Microbiol 38:2156–2161
    [Google Scholar]
  12. Okuda J., Ishibashi M., Hayakawa E., Nishino T., Takeda Y., Mukhopadhyay A. K., Garg S., Bhattacharya S. K., Nair G. B., Nishibuchi M. 1997; Emergence of a unique O3 : K6 clone of Vibrio parahaemolyticus in Calcutta, India, and isolation of strains from the same clonal group from Southeast Asian travelers arriving in Japan. J Clin Microbiol 35:3150–3155
    [Google Scholar]
  13. Tenover F. C., Arbeit R. D., Goering R. V., Mickelsen P. A., Murray B. E., Persing D. H., Swaminathan B. 1995; Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 33:2233–2239
    [Google Scholar]
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