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Volume 53, Issue 10

Sorting a phage display library against biomaterial Free

Abstract

A phage display library made from DNA was sorted against a central venous catheter (CVC) that had been removed from a patient 2 days after insertion. After the first panning, approximately 50 % of the clones encoded proteins known to interact with mammalian proteins. After the second and third pannings, fibrinogen-binding and β-glycoprotein I (β-GPI)-binding phage particles were clearly dominating. Proteins adsorbed to different CVCs were investigated using specific antibodies. Among the proteins probed for, fibrinogen was most abundant, but, interestingly, β-GPI was also detected on all tested CVCs.

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2004-10-01
2024-05-02
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Sorting a Staphylococcus aureus phage display library against ex vivo biomaterial
J. Med. Microbiol. 53, 945 (2004); https://doi.org/10.1099/jmm.0.45638-0
/content/journal/jmm/10.1099/jmm.0.45638-0
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