1887

Abstract

strains isolated between February 2002 and March 2004 from 30 episodes of infection in 26 patients at Vienna University Hospital were characterized. Twenty-four of the episodes occurred within a 7 month period, suggesting they were outbreak-related, although no common source of infection was identified. The isolates were assayed using PCR to confirm species identification. Random amplification of polymorphic DNA (RAPD) typing classified the isolates into four distinct genotypes: A/I, B/II, C/III and D/IV (15, 13, 1 and 1 isolates, respectively). API 20NE, VITEK Gram-negative Identification Card plus (GNI+) and VITEK Gram Negative Bacillus Identification (GNB) yielded negative or no acceptable biochemical profile for 4, 11 and 11 isolates, respectively. None of the isolates acidified -arabitol or mannitol. Two isolates (7 %) were positive for nitrate reduction. All 30 isolates were resistant to desferrioxamine, and 29 were able to grow on BCSA. The most active compounds were ciprofloxacin and cefepime, whilst only the genotype D/IV isolate was sensitive to gentamicin and amikacin (the remaining 29 isolates being resistant to both).

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2005-01-01
2024-03-28
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References

  1. Boutros N., Gonullu N., Casetta A., Guibert M., Ingrand D., Lebrun L. 2002; Ralstonia pickettii traced in blood culture bottles. J Clin Microbiol 40:2666–2667 [CrossRef]
    [Google Scholar]
  2. Chen J. S., Witzmann K. A., Spilker T., Fink R. J., LiPuma J. J. 2001; Endemicity and inter-city spread of Burkholderia cepacia genomovar III in cystic fibrosis. J Pediatr 139:643–649 [CrossRef]
    [Google Scholar]
  3. Coenye T., Goris J., Spilker T., Vandamme P., LiPuma J. J. 2002a; Characterization of unusual bacteria isolated from respiratory secretions of cystic fibrosis patients and description of Inquilinus limosus gen.nov.,sp. nov. . J Clin Microbiol 40:2062–2069 [CrossRef]
    [Google Scholar]
  4. Coenye T., Vandamme P., LiPuma J. J. 2002b; Infection by Ralstonia species in cystic fibrosis patients: identification of R.pickettii and R. mannitolilytica by polymerase chain reaction. Emerg Infect Dis 8:692–696 [CrossRef]
    [Google Scholar]
  5. De Baere T., Steyaert S., Wauters G., Des Vos P., Goris J., Coenye T., Suyama T., Verschraegen G., Vaneechoutte M. 2001; Classification of Ralstonia pickettii biovar 3/`thomasii’ strains; (Pickett 1994; and of new isolates related to nosocomial recurrent meningitis as Ralstonia mannitolytica sp.nov. Int J Syst Evol Microbiol 51:547–558
    [Google Scholar]
  6. Denton M., Kerr K. G. 1998; Microbiological and clinical aspects of infection associated with Stenotrophomonas maltophilia . Clin Microbiol Rev 11:57–80
    [Google Scholar]
  7. Henry D. A., Campbell M. E., LiPuma J. J., Speert D. P. 1997; Identification of Burkholderia cepacia isolates from patients with cystic fibrosis and use of a simple new selective medium. J Clin Microbiol 35:614–619
    [Google Scholar]
  8. Henry D., Campbell M., McGimpsey C., Clarke A., Louden L., Burns J. L., Roe M. H., Vandamme P., Speert D. 1999; Comparison of isolation media for recovery of Burkholderia cepacia complex from respiratory secretions of patients with cystic fibrosis. J Clin Microbiol 37:1004–1007
    [Google Scholar]
  9. Henry D. A., Mahenthiralingam E., Vandamme P., Coenye T., Speert D. P. 2001; Phenotypic methods for determining genomovar status of the Burkholderia cepacia complex. J Clin Microbiol 39:1073–1078 [CrossRef]
    [Google Scholar]
  10. Kiska D. L., Kerr A., Jones M. C. & 7 other authors; 1996; Accuracy of four commercial systems for identification of Burkholderia cepacia and other Gram-negative nonfermenting bacilli recovered from patients with cystic fibrosis. J Clin Microbiol 34:886–891
    [Google Scholar]
  11. Labarca J. A., Trick W. E., Peterson C. L., Carson L. A., Holt S. C., Arduino M. J., Meylan M., Mascola L., Jarvis W. R. 1999; A multistate nosocomial outbreak of Ralstonia pickettii colonization associated with an intrinsically contaminated respiratory care solution. Clin Infect Dis 29:1281–1286 [CrossRef]
    [Google Scholar]
  12. Laffineur K., Janssens M., Charlier J., Avesani V., Wauters G., Delmee M. 2002; Biochemical and susceptibility tests useful for identification of nonfermenting Gram-negative rods. J Clin Microbiol 40:1085–1087 [CrossRef]
    [Google Scholar]
  13. Lindsay J. A., Riley T. V. 1991; Susceptibility to desferrioxamine: a new test for the identification of Staphylococcus epidermidis . J Med Microbiol 35:45–48 [CrossRef]
    [Google Scholar]
  14. Mahenthiralingam E., Campbell M. E., Foster J., Lam J. S., Speert D. P. 1996; Random amplified polymorphic DNA typing of Pseudomonas aeruginosa isolates recovered from patients with cystic fibrosis. J Clin Microbiol 34:1129–1135
    [Google Scholar]
  15. Maroye P., Doermann H. P., Rogues A. M., Gachie J. P., Megraud F. 2000; Investigation of an outbreak of Ralstonia pickettii in a paediatric hospital by RAPD. J Hosp Infect 44:267–272 [CrossRef]
    [Google Scholar]
  16. Moissenet D., Bidet P., Garbarg-Chenon A., Arlet G., Vu-Thien H. 2001; Ralstonia paucula (Formerly CDC group IV c-2): unsuccessful strain differentiation with PCR-based methods, study of the 16S-23S spacer of the rRNA operon, and comparison with other Ralstonia species (R.eutropha , R. pickettii , R. gilardii ,and R. solanacearum ). J Clin Microbiol 39:381–384 [CrossRef]
    [Google Scholar]
  17. Pan H. J., Teng L. J., Tzeng M. S., Chang S. C., Ho S. W., Luh K. T., Hsieh W. C. 1992; Identification and typing of Pseudomonas pickettii during an episode of nosocomial outbreak. Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi 25:115–123 (in Chinese
    [Google Scholar]
  18. Phillips I., Eykyn S., Laker M. 1972; Outbreak of hospital infection caused by contaminated autoclaved fluids. Lancet i:1258–1260
    [Google Scholar]
  19. Renders N., Romling Y., Verbrugh H., van Belkum A. 1996; Comparative typing of Pseudomonas aeruginosa by random amplification of polymorphic DNA or pulsed-field gel electrophoresis of DNA macrorestriction fragments. J Clin Microbiol 34:3190–3195
    [Google Scholar]
  20. Saiman L., Siegel J. 2004; Infection control in cystic fibrosis. Clin Microbiol Rev 17:57–71 [CrossRef]
    [Google Scholar]
  21. Vandamme P., Goris J., Coenye T., Hoste B., Janssens D., Kersters K., De Vos P., Falsen E. 1999; Assignment of Centers for Disease Control group IVc-2 to the genus Ralstonia as Ralstonia paucula sp.nov. Int J Syst Bacteriol 49:663–669 [CrossRef]
    [Google Scholar]
  22. Vaneechoutte M., De Baere T., Wauters G., Steyaert S., Claeys G., Vogelaers D., Verschraegen G. 2001; One case each of recurrent meningitis and hemoperitoneum infection with Ralstonia mannitolilytica . J Clin Microbiol 39:4588–4590 [CrossRef]
    [Google Scholar]
  23. Wauters G., Claeys G., Verschraegen G., De Baere T., Vandecruys E., Van Simaey L., De Ganck C., Vaneechoutte M. 2001; Case of catheter sepsis with Ralstonia gilardii in a child with acute lymphoblastic leukemia. J Clin Microbiol 39:4583–4584 [CrossRef]
    [Google Scholar]
  24. Wright R. M., Moore J. E., Shaw A. & 8 other authors; 2001; Improved cultural detection of Burkholderia cepacia from sputum in patients with cystic fibrosis. J Clin Pathol 54:803–805 [CrossRef]
    [Google Scholar]
  25. Yabuuchi E., Kosako Y., Yano I., Hotta H., Nishiuchi Y. 1995; Transfer of two Burkholderia and an Alcaligenes species to Ralstonia gen.nov.: proposal of Ralstonia pickettii (Ralston, Palleroni and Doudoroff 1973) comb.nov., Ralstonia solanacearum (Smith 1896) comb. nov. and Ralstonia eutropha (Davis 1969) comb. nov.. Microbiol Immunol 39:897–904 [CrossRef]
    [Google Scholar]
  26. Yoneyama A., Yano H., Hitomi S., Okuzumi K., Suzuki R., Kimura S. 2000; Ralstonia pickettii colonization of patients in an obstetric ward caused by a contaminated irrigation system. J Hosp Infect 46:79–80 [CrossRef]
    [Google Scholar]
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