@article{mbs:/content/journal/jmm/10.1099/jmm.0.45766-0, author = "Nelson, Michelle and Prior, Joann L and Lever, M Stephen and Jones, Helen E and Atkins, Timothy P and Titball, Richard W", title = "Evaluation of lipopolysaccharide and capsular polysaccharide as subunit vaccines against experimental melioidosis", journal= "Journal of Medical Microbiology", year = "2004", volume = "53", number = "12", pages = "1177-1182", doi = "https://doi.org/10.1099/jmm.0.45766-0", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.45766-0", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", abstract = " Burkholderia pseudomallei is the causative agent of melioidosis, which is a major cause of morbidity and mortality in endemic regions. Currently there is no human vaccine against melioidosis. In this study, LPS or capsular polysaccharide was used to immunize BALB/c mice. The different polysaccharide antigens induced antibody responses. Mice vaccinated with LPS developed predominantly IgM and IgG3 responses. Contrastingly, mice vaccinated with capsular polysaccharide developed a predominantly IgG2b response. After immunization, mice were challenged by the intra-peritoneal route and an increased mean time to death was observed compared with unvaccinated controls. Immunization with LPS provided an optimal protective response. Mice challenged by the aerosol route showed a small increase in the mean time to death compared with the unvaccinated controls. The passive transfer of antigen from immunized into naïve mice provided protection against a subsequent challenge. This study is the first time antigens protective by active immunization have been identified and suggests that polysaccharides have potential as vaccine candidates against melioidosis.", }