Evaluation of lipopolysaccharide and capsular polysaccharide as subunit vaccines against experimental melioidosis Nelson, Michelle and Prior, Joann L and Lever, M Stephen and Jones, Helen E and Atkins, Timothy P and Titball, Richard W,, 53, 1177-1182 (2004), doi = https://doi.org/10.1099/jmm.0.45766-0, publicationName = Microbiology Society, issn = 0022-2615, abstract= Burkholderia pseudomallei is the causative agent of melioidosis, which is a major cause of morbidity and mortality in endemic regions. Currently there is no human vaccine against melioidosis. In this study, LPS or capsular polysaccharide was used to immunize BALB/c mice. The different polysaccharide antigens induced antibody responses. Mice vaccinated with LPS developed predominantly IgM and IgG3 responses. Contrastingly, mice vaccinated with capsular polysaccharide developed a predominantly IgG2b response. After immunization, mice were challenged by the intra-peritoneal route and an increased mean time to death was observed compared with unvaccinated controls. Immunization with LPS provided an optimal protective response. Mice challenged by the aerosol route showed a small increase in the mean time to death compared with the unvaccinated controls. The passive transfer of antigen from immunized into naïve mice provided protection against a subsequent challenge. This study is the first time antigens protective by active immunization have been identified and suggests that polysaccharides have potential as vaccine candidates against melioidosis., language=, type=