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Volume 54, Issue 3

Monochrome LightCycler PCR assay for detection and quantification of five common species of and Free

Abstract

Invasive fungal pathogens, especially in immunocompromised hosts, can result in life-threatening infections. Current laboratory/radiological methods for fungal identification are time-consuming and lack sensitivity and specificity. A monochrome, multiplex, real-time PCR assay for the identification and quantification of , , , and is described here. Detection of each of these fungi was specific and demonstrated 100 % concordance with biochemical/culture identification in all 60 isolates tested. Samples from 16 febrile neutropenic patients with haematological malignancies were also analysed and the utility of the assay in clinical samples was reconfirmed without false-negative results. The sensitivity of this assay was 0.1 pg fungal genomic DNA, corresponding to three cells, for , , and , and 0.01 pg fungal genomic DNA, i.e. less than one cell, for . The analysis allows a low-cost, simple, rapid and sensitive alternative for clinical identification and quantification of these five common fungal species.

  • Received:
  • Accepted:
  • Published Online:
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2005-03-01
2024-04-28
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Monochrome LightCycler PCR assay for detection and quantification of five common species of Candida and Aspergillus
J. Med. Microbiol. 54, 243 (2005); https://doi.org/10.1099/jmm.0.45856-0
/content/journal/jmm/10.1099/jmm.0.45856-0
/content/journal/jmm/10.1099/jmm.0.45856-0
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