1887

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Volume 54, Issue 3

heat-shock protein 60 induces production of the pro-inflammatory cytokine IL8 in monocytic cells Free

Abstract

Interleukin 8 (IL8) is usually produced in both epithelial and monocytic cells during bacterial infections, causing inflammation. induces production of IL8 from gastric epithelial cells via its pathogenicity island (cag PAI) system, LPS and outer-membrane protein. In some bacteria, heat-shock protein 60 (HSP60) also elicits a strong pro-inflammatory response in cells of the innate immune system. Three recombinant HSP60 (rHSP60) proteins of different sizes were produced and one of these was used to raise two monoclonal antibodies (2E7 and 7B5). IL8 production was found to be induced in cultured monocytic cells treated with cells or rHSP60 proteins, as measured by ELISA, and the amount of IL8 produced was dose-dependent. Pre-incubation of cells or rHSP60 preparations with the antibody 2E7 significantly inhibited IL8 production from monocytic cells. These results indicated that HSP60 is closely associated with IL8 production in monocytic cells.

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© Microbiology Society
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2005-03-01
2024-04-20
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Helicobacter pylori heat-shock protein 60 induces production of the pro-inflammatory cytokine IL8 in monocytic cells
J. Med. Microbiol. 54, 225 (2005); https://doi.org/10.1099/jmm.0.45871-0
/content/journal/jmm/10.1099/jmm.0.45871-0
/content/journal/jmm/10.1099/jmm.0.45871-0
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