Development of a routine laboratory direct detection system of staphylococcal enterotoxin genes
Nakayama, Akifumi and Okayama, Akiko and Hashida, Misao and Yamamoto, Yasuzumi and Takebe, Hisakatsu and Ohnaka, Takashi and Tanaka, Tomoyuki and Imai, Shunsuke,, 55, 273-277 (2006),
doi = https://doi.org/10.1099/jmm.0.46027-0,
publicationName = Microbiology Society,
issn = 0022-2615,
abstract= A novel direct detection system has been developed for eight staphylococcal enterotoxin (SE)-encoding genes (sea, seb, sec, sed, see, seg, seh and sei) in milk. Specific detection by real-time PCR was successful for all SE-encoding genes in the reference strains. Furthermore, a novel DNA-preparation method with good reproducibility [coefficients of variation 0·31, 0·99 and 1·21 % at 106, 104 and 102 c.f.u. (ml milk sample)−1, respectively] was developed to overcome PCR inhibition in the milk samples. The combination of this DNA-preparation method and real-time PCR resulted in high sensitivity [between 1·1×102 and 1·0×104 c.f.u. (ml milk sample)−1] and allowed the completion of the entire procedure within 4 h. Results of an evaluation of this method for the detection of SE-encoding genes using known outbreak milk samples produced results showing good correspondence with the reversed passive latex agglutination assay. In addition, this newly developed system can be applied to clinical samples such as faeces and vomit. Consequently, the system should be useful in the routine direct detection of SE-encoding genes in food-borne-poisoning samples.,
language=,
type=