1887

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Volume 54, Issue 12

Rapid and simple detection of genes by multiplex PCR assay Free

Abstract

A novel multiplex PCR assay is described (CTX-Mplex PCR) that allows rapid detection of genes and discrimination between groups 1, 2, 9 and 25/26. The specificity and sensitivity of the assay were evaluated with 10 control strains and then applied to 62 clinical isolates. The multiplex PCR detected and classified genes with 100 % accuracy. The utilization of a denaturing HPLC WAVE system to size the PCR products automatically from the multiplex PCR enhances the assay by saving time and costs.

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© Microbiology Society
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/content/journal/jmm/10.1099/jmm.0.46160-0
2005-12-01
2024-04-19
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Rapid and simple detection of blaCTX−M genes by multiplex PCR assay
J. Med. Microbiol. 54, 1183 (2005); https://doi.org/10.1099/jmm.0.46160-0
/content/journal/jmm/10.1099/jmm.0.46160-0
/content/journal/jmm/10.1099/jmm.0.46160-0
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