Rapid and simple detection of blaCTX−M genes by multiplex PCR assay
Xu, Li and Ensor, Vicki and Gossain, Savita and Nye, Kathy and Hawkey, Peter,, 54, 1183-1187 (2005),
doi = https://doi.org/10.1099/jmm.0.46160-0,
publicationName = Microbiology Society,
issn = 0022-2615,
abstract= A novel multiplex PCR assay is described (CTX-Mplex PCR) that allows rapid detection of bla CTX−M genes and discrimination between groups 1, 2, 9 and 25/26. The specificity and sensitivity of the assay were evaluated with 10 control strains and then applied to 62 clinical isolates. The multiplex PCR detected and classified bla CTX−M genes with 100 % accuracy. The utilization of a denaturing HPLC WAVE system to size the PCR products automatically from the multiplex PCR enhances the assay by saving time and costs.,
language=,
type=