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Volume 55, Issue 4

Characterization of extended-spectrum -lactamase (ESBL)-producing Kuwait and UK strains identified by the Vitek system, and subsequent comparison of the Vitek system with other commercial ESBL-testing systems using these strains Free

Abstract

Two hundred and fifty-one unique patient isolates of (123), (114), (7), (5) and (2), flagged as extended-spectrum -lactamase (ESBL) positive by the Vitek system (GNS-526 card), were collected. These strains were isolated from a variety of clinical specimens submitted to the clinical bacteriology laboratories of the Royal Infirmary of Edinburgh (RIE), Edinburgh, UK (and associated GP practices), Hairmyers Hospital, Glasgow, UK, and the Amiri and Farwania Hospitals, Kuwait. Of the 101 RIE strains tested, 15 strains were found to be ESBL negative by Etest ESBL strips. On retesting the 15 strains with the Vitek GNS-532 card, 14 were found to be ESBL negative, despite being originally flagged as ESBL positive. The remaining 236 ESBL-producing strains were also subjected to the double disc-diffusion (DDD) technique for the detection of ESBLs. Of these, two were false negatives by Etest ESBL test strips (using both cefotaxime and ceftazidime strips), and 38 were false negatives by the DDD method. The Etest false-negative ESBL-producing strains of were positive by DDD. Technically, the Vitek method was the least demanding method to perform, as it was an integral part of the routine susceptibility test card. Etest strips were reliable, but were the most expensive of all the techniques used. The DDD test, while relatively inexpensive, was technically subjective, and in our hands, seven of the ESBL-positive strains that were confirmed by the other two techniques were not detected. Despite the false-positive ESBL-producing strains, the Vitek susceptibility card with its integral ESBL test offers the clinical laboratory a valuable and quick option to screen for ESBL-producing spp. and as part of the routine laboratory methodology.

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Keyword(s): DDD, double disc-diffusion , ESBL, extended-spectrum β-lactamase and RIE, Royal Infirmary of Edinburgh
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2006-04-01
2024-04-27
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Characterization of extended-spectrum β-lactamase (ESBL)-producing Kuwait and UK strains identified by the Vitek system, and subsequent comparison of the Vitek system with other commercial ESBL-testing systems using these strains
J. Med. Microbiol. 55, 417 (2006); https://doi.org/10.1099/jmm.0.46177-0
/content/journal/jmm/10.1099/jmm.0.46177-0
/content/journal/jmm/10.1099/jmm.0.46177-0
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