Detection of mixed infections with Mycobacterium lentiflavum and Mycobacterium avium by molecular genotyping methods Suffys, Philip and da Silva Rocha, Adalgiza and Brandão, Adeilton and Vanderborght, Bart and Mijs, Wouter and Jannes, Geert and Mello, Fernanda C. Q. and Pedro, Heloisa da Silveira Paro and de Souza Fonseca, Leila and de Oliveira, Rosângela Siqueira and Cardoso Leão, Sylvia and Féres Saad, Maria Helena,, 55, 127-131 (2006), doi = https://doi.org/10.1099/jmm.0.46218-0, publicationName = Microbiology Society, issn = 0022-2615, abstract= Three mycobacterial isolates, one from the blood of an HIV-infected patient and two consecutive isolates from a woman with unknown HIV status, had been identified as belonging to the Mycobacterium avium complex by conventional procedures. In both patients, using genetic analysis procedures such as PCR–restriction enzyme analysis (PRA) of the hsp65 gene, a commercially available reverse hybridization-based assay (INNO-LiPA mycobacteria) and/or sequencing analysis of the 16S–23S internal transcribed spacer (ITS), the presence of Mycobacterium lentiflavum was also demonstrated. At the time of detection, both cases were also infected with M. avium, suggesting an underestimation of infection with M. lentiflavum and co-infection with different Mycobacterium species., language=, type=