RT Journal Article SR Electronic(1) A1 Suffys, Philip A1 da Silva Rocha, Adalgiza A1 Brandão, Adeilton A1 Vanderborght, Bart A1 Mijs, Wouter A1 Jannes, Geert A1 Mello, Fernanda C. Q. A1 Pedro, Heloisa da Silveira Paro A1 de Souza Fonseca, Leila A1 de Oliveira, Rosângela Siqueira A1 Cardoso Leão, Sylvia A1 Féres Saad, Maria HelenaYR 2006 T1 Detection of mixed infections with Mycobacterium lentiflavum and Mycobacterium avium by molecular genotyping methods JF Journal of Medical Microbiology, VO 55 IS 1 SP 127 OP 131 DO https://doi.org/10.1099/jmm.0.46218-0 PB Microbiology Society, SN 1473-5644, AB Three mycobacterial isolates, one from the blood of an HIV-infected patient and two consecutive isolates from a woman with unknown HIV status, had been identified as belonging to the Mycobacterium avium complex by conventional procedures. In both patients, using genetic analysis procedures such as PCR–restriction enzyme analysis (PRA) of the hsp65 gene, a commercially available reverse hybridization-based assay (INNO-LiPA mycobacteria) and/or sequencing analysis of the 16S–23S internal transcribed spacer (ITS), the presence of Mycobacterium lentiflavum was also demonstrated. At the time of detection, both cases were also infected with M. avium, suggesting an underestimation of infection with M. lentiflavum and co-infection with different Mycobacterium species., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.46218-0