1887

Abstract

is the most common pathogen causing dermatophytosis. Molecular strain-typing methods have recently been developed to tackle epidemiological questions and the problem of relapse following treatment. A total of 67 strains of were screened for genetic variation by randomly amplified polymorphic DNA (RAPD) analysis, with two primers, 5′-d[GGTGCGGGAA]-3′ and 5′-d[CCCGTCAGCA]-3′, as well as by subrepeat element analysis of the nontranscribed spacer of rDNA, using the repetitive subelements TRS-1 and TRS-2. A total of 12 individual patterns were recognized with the first primer and 11 with the second. Phylogenetic analysis of the RAPD products showed a high degree of similarity (>90 %) among the epidemiologically related clinical isolates, while the other strains possessed 60 % similarity. Specific amplification of TRS-1 produced three strain-characteristic banding patterns (PCR types); simple patterns representing one copy of TRS-1 and two copies of TRS-2 accounted for around 85 % of all isolates. It is concluded that molecular analysis has important implications for epidemiological studies, and RAPD analysis is especially suitable for molecular typing in

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.46236-0
2006-04-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jmm/55/4/429.html?itemId=/content/journal/jmm/10.1099/jmm.0.46236-0&mimeType=html&fmt=ahah

References

  1. Baeza L. C., Mendes-Giannini M. J. S. 2004; Strain differentiation of Trichophyton rubrum by random amplification of polymorphic DNA (RAPD). Rev Inst Med Trop Sao Paulo 46:339–341 [CrossRef]
    [Google Scholar]
  2. Chong P. P., Lee Y. L., Tan B. C., Ng K. P. 2003; Genetic relatedness of Candida strains isolated from women with vaginal candidiasis in Malaysia. J Med Microbiol 52:657–666 [CrossRef]
    [Google Scholar]
  3. Del Sal G., Manfioletti G., Schneider C. 1989; The CTAB-DNA precipitation method: a common mini-scale preparation of template DNA from phagemids, phages or plasmids suitable for sequencing. Biotechniques 7:514–519
    [Google Scholar]
  4. Ellsworth D. L., Rittenhouse K. D., Honeycutt R. L. 1993; Artifactual variation in randomly amplified polymorphic DNA banding patterns. Biotechniques 14:214–217
    [Google Scholar]
  5. Evans E. G. 1998; Causative pathogens in onychomycosis and the possibility of treatment resistance: a review. J Am Acad Dermatol 38:S32–S56 [CrossRef]
    [Google Scholar]
  6. Faergemann J., Correia O., Nowicki R., Ro B.-I. 2005; Genetic predisposition – understanding underlying mechanisms of onychomycosis. J Eur Acad Dermatol Venereol 19 (suppl. 1):17–19 [CrossRef]
    [Google Scholar]
  7. Gräser Y., Kuijpers A. F. A., Presber W., Hoog G. S. 2000; Molecular taxonomy of the Trichophyton rubrum complex. J Clin Microbiol 38:3329–3336
    [Google Scholar]
  8. Gupta A. K., Kohli Y., Summerbell R. C. 2001; Variation in restriction fragment length polymorphisms among serial isolates from patients with Trichophyton rubrum infection. J Clin Microbiol 39:3260–3266 [CrossRef]
    [Google Scholar]
  9. Harmsen D., Schwinn A., Weig M., Brocker E. B., Heesemann J. 1995; Phylogeny and dating of some pathogenic keratinophilic fungi using small subunit ribosomal RNA. J Med Vet Mycol 33:299–303 [CrossRef]
    [Google Scholar]
  10. Jackson C. J., Barton R. C., Kelly S. L., Evans E. G. V. 2000; Strain identification of Trichophyton rubrum by specific amplification of subrepeat elements in the ribosomal DNA nontranscribed spacer. J Clin Microbiol 38:4527–4534
    [Google Scholar]
  11. Kac G., Bougnoux M. E., Feuilhade De Chauvin M., Sené S., Derouin F. 1999; Genetic diversity among Trichophyton mentagrophytes isolates using random amplified polymorphic DNA method. Br J Dermatol 140:839–844 [CrossRef]
    [Google Scholar]
  12. Kamiya A., Kikuchi A., Tomita Y., Kanbe T. 2004; PCR and PCR-RFLP techniques targeting the DNA topoisomerase II gene for rapid clinical diagnosis of the etiologic agent of dermatophytosis. J Dermatol Sci 34:35–38 [CrossRef]
    [Google Scholar]
  13. Liu D., Coloe S., Pedersen J., Baird R. 1996; Use of arbitrarily primed polymerase chain reaction to differentiate Trichophyton dermatophytes. FEMS Microbiol Lett 136:147–150 [CrossRef]
    [Google Scholar]
  14. Rad M. M., Jackson C., Barton R. C., Evans E. G. V. 2005; Single strains of Trichophyton rubrum in cases of tinea pedis. J Med Microbiol 54:725–726 [CrossRef]
    [Google Scholar]
  15. Soll D. R. 2000; The ins and outs of DNA fingerprinting the infectious fungi. J Clin Microbiol 13:332–370 [CrossRef]
    [Google Scholar]
  16. Summerbell R. C., Kane J. 1997; Physiological and other special tests for identifying dermatophytes. In Laboratory Handbook of Dermatophytes pp  45–79 Edited by Kane J., Summerbell R. Belmont, CA: Star Publishing;
    [Google Scholar]
  17. Summerbell R. C., Li A., Haugland R. 1997; What constitutes a functional species in the asexual dermatophytes?. Microbiol Cult Collect 13:29–37
    [Google Scholar]
  18. Summerbell R. C., Haugland R. A., Li A., Gupta A. K. 1999; rRNA gene internal transcribed spacer 1 and 2 sequences of asexual, anthropophilic dermatophytes related to Trichophyton rubrum . J Clin Microbiol 37:4005–4011
    [Google Scholar]
  19. Yazdanparast Y., Jackson C. J., Barton R. C., Evans E. G. V. 2003; Molecular strain typing of Trichophyton rubrum indicates multiple strain involvement in onychomycosis. Br J Dermatol 148:51–54 [CrossRef]
    [Google Scholar]
  20. Zaias N., Tosti A., Rebell G. & 8 other authors; 1996; Autosomal dominant pattern of distal subungual onychomycosis caused by Trichophyton rubrum . J Am Acad Dermatol 34:302–304 [CrossRef]
    [Google Scholar]
  21. Zhong Z., Li R., Li D., Wang D. 1997; Typing of common dermatophytes by random amplification of polymorphic DNA. Jpn J Med Mycol 38:239–246 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.46236-0
Loading
/content/journal/jmm/10.1099/jmm.0.46236-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error