1887

Abstract

The distribution of 19 major virulence genes and the presence of plasmids were surveyed in 141 serogroup (SG) 1 isolates from patients and water in Queensland, Australia. The results showed that 16 of the virulence genes examined were present in all isolates, suggesting that they are life-essential genes for isolates in the environment and host cells. The 65 kb pathogenicity island identified originally in strain Philadelphia-1 was detected more frequently in isolates from water (44.2 %) than in those from patients (2.7 %), indicating that the 65 kb DNA fragment may aid the survival of in the sampled environment. However, the low frequency of the 65 kb fragment in isolates from patients suggests that the pathogenicity island may not be necessary for to cause disease. Plasmids were not detected in the SG1 isolates from patients or water studied. There was an association of both and with the virulent and predominant genotype detected by amplified fragment length polymorphism, termed AF1, whereas the avirulent common isolate from water termed AF16 did not have or genes, with the exception of one isolate with . It was found that a PCR detection test strategy with and as pathogenesis markers would be useful for determining the infection potential of an isolate.

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2006-08-01
2024-03-28
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