@article{mbs:/content/journal/jmm/10.1099/jmm.0.46376-0, author = "Iqbal, Jamshaid and Sher, Ali", title = "Determination of the prevalence of lymphatic filariasis among migrant workers in Kuwait by detecting circulating filarial antigen", journal= "Journal of Medical Microbiology", year = "2006", volume = "55", number = "4", pages = "401-405", doi = "https://doi.org/10.1099/jmm.0.46376-0", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.46376-0", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", keywords = "CFA, circulating filarial antigen", keywords = "ICT, immunochromatographic test", keywords = "AU, antigen units", keywords = "NFB, nucleopore-filtered blood", keywords = "mf, microfilariae", keywords = "ML, maximum likelihood", abstract = "The main objective of this study was to determine the prevalence of filarial infection among migrant workers in Kuwait. The study was conducted from April 2000 to November 2003. A total of 1050 migrant workers (>90 % from the Indian subcontinent) from filarial endemic countries and 260 individuals residing in Kuwait as controls (50 healthy Kuwaiti blood donors, 50 microfilaria-negative individuals from endemic areas and 160 patients with other parasitic infections) were screened for filarial infection. All specimens were tested for microfilaraemia by microscopy of nucleopore-filtered blood (NFB) and detection of circulating filarial antigen (CFA) by an immunochromatographic test (ICT) and the TropBio assay. The overall prevalence of filarial antigenaemia was 18·3 % (192 individuals) using the ICT and 20·3 % (213 individuals) using the TropBio assay. Thirty-two cases (3 %) of Wuchereria bancrofti were detected by microscopy and the mean microfilaria count in these cases was 816 microfilariae ml−1. CFA was detected only in two of the 260 control subjects. Statistical analysis to calculate the sensitivity, specificity and prevalence of infection was carried out using maximum-likelihood statistical methods. The overall sensitivity and specificity of the ICT and TropBio assay to detect CFA were comparable. Compared with NFB microscopy, the sensitivity of the ICT was 93·8 % and specificity ranged from 84 to 100 %. The sensitivity and specificity of the TropBio assay were 90·1 and 100 %, respectively. However, the ICT failed to detect CFA in two cases with a microfilarial load of <20 microfilariae ml−1. In conclusion, the prevalence of filarial infection among the migrant workers in Kuwait was 18·3 % as determined by the ICT.", }