1887

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Volume 55, Issue 10

Detection of antibodies against culture filtrate protein-10 in leprosy patients Free

Abstract

The prevalence of IgG antibodies against recombinant culture filtrate protein-10 (rCFP-10) was investigated in serum samples from 56 leprosy patients, 15 tuberculosis (TB) patients, 14 other skin-diseased patients and 20 healthy subjects. On classifying the patients into bacterial index (BI)-positive and BI-negative groups, the assay showed 83.3 % (15/18) sensitivity for detection of BI-positive leprosy patients. On the other hand, the sensitivity for detection of BI-negative patients was 18.4 % (7/38). None of the 15 TB patients and 14 other skin-diseased patients was positive; however, only one out of 20 healthy individuals was positive, indicating that antibody response to culture filtrate protein-10 (CFP-10) was highly specific (98.0 %; 48/49). Statistically, the performance of the CFP-10-based assay was found to be comparable (>0.05) with that of an anti-phenolic glycolipid-I (PGL-I) antibody-detecting assay. Thus, CFP-10 is potentially a specific antigen for measuring antibody response in BI-positive leprosy patients. Being a secreted antigen, CFP-10 may act as a marker for the viability of inside the host, and hence its serological potential is worth exploring for application in monitoring the response of patients with BI-positive leprosy (a highly infectious form) during the course of chemotherapy. When comparing the bacteriological and serological results, an agreement of 82.1 % showed that seropositivity to CFP-10 corresponded well with bacteriological criteria. Hence, CFP-10 seems to be a suitable antigen for classification of leprosy patients into BI-positive and BI-negative groups.

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Keyword(s): BI, bacterial index , CFP-10, culture filtrate protein-10 , MDT, multidrug therapy , PGL-I, phenolic glycolipid-I , rCFP-10, recombinant CFP-10 , ROC curve, receiver operator characteristic curve and TB, tuberculosis
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2006-10-01
2024-04-19
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Detection of antibodies against Mycobacterium leprae culture filtrate protein-10 in leprosy patients
J. Med. Microbiol. 55, 1337 (2006); https://doi.org/10.1099/jmm.0.46587-0
/content/journal/jmm/10.1099/jmm.0.46587-0
/content/journal/jmm/10.1099/jmm.0.46587-0
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