1887

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Volume 55, Issue 12

translocates throughout the digestive tract in asymptomatic sheep Free

Abstract

Ruminants are fed forage which is often contaminated with , and frequently shed with their faeces. The present study was designed to localize the sites of infection in the digestive tract concomitant with faecal excretion in a sheep model. Ten -free sheep were inoculated with a dose of 10 c.f.u. of a pathogenic strain. received by two of the ten animals were radiolabelled with indium oxine. The dissemination of the was assessed by imaging, by culture of bacteria in the faeces, organs and digesta samples taken at slaughter on days 1, 2, 6, 10 and 14 post-inoculation, and by measuring gamma radioactivity of samples on day 6. It was shown that spread through the entire volume of the forestomachs within 4 h, and through the whole gastrointestinal tract (GIT) within 24 h. Faecal shedding of lasted 10 days. Rumen, duodenum, jejunum, ileum, caecum and colon walls and digesta, mesenteric lymph nodes, liver and spleen were temporarily infected. However, persisted for at least 14 days in rumen digesta and retropharyngeal lymph nodes, and at a relatively high level (1×10 c.f.u. g) in palatine tonsils. These findings suggest that can translocate from all parts of the GIT, with the rumen digesta, but not the gallbladder, serving as a reservoir. The results indicate that brief and low-level faecal excretion of is concomitant with a transitory asymptomatic infection in sheep.

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Keyword(s): GIT, gastrointestinal tract , MPN, most probable number , PI, post-inoculation and ROI, region of interest
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2006-12-01
2024-05-01
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Listeria monocytogenes translocates throughout the digestive tract in asymptomatic sheep
J. Med. Microbiol. 55, 1717 (2006); https://doi.org/10.1099/jmm.0.46709-0
/content/journal/jmm/10.1099/jmm.0.46709-0
/content/journal/jmm/10.1099/jmm.0.46709-0
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