1887

Abstract

Molecular typing and virulence markers were used to evaluate the genetic profiles and virulence potential of 106 strains. Of these strains, 71 were bio-serotype 4/O : 3, isolated from human and animal clinical material, and 35 were of biotype 1A or 2 and of diverse serotypes, isolated from food in Brazil between 1968 and 2000. Drug resistance was also investigated. All the strains were resistant to three or more drugs. The isolates showed a virulence-related phenotype in the aesculin, pyrazinamidase and salicin tests, except for the food isolates, only two of which were positive for these tests. For the other phenotypic virulence determinants (autoagglutination, Ca dependence and Congo red absorption), the strains showed a diverse behaviour. The , and A genes were detected in all human and animal strains, while all the food isolates were positive for , and 3 % of them positive for and A. The presence of F was variable in the three groups of strains. The strains were better discriminated by PFGE than by enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). A higher genomic similarity was observed among the 4/O : 3 strains, isolated from human and animal isolates, than among the food strains, with the exception of two food strains possessing the virulence genes and grouped close to the 4/O : 3 strains by ERIC-PCR. Unusually, the results revealed the virulence potential of a bio-serotype 1A/O : 10 strain, suggesting that food contaminated with biotype 1A may cause infection. This also suggests that ERIC-PCR may be used as a tool to reveal clues about the virulence potential of strains. Furthermore, the results also support the hypothesis that animals may act as reservoirs of for human infections in Brazil, an epidemiological aspect that has not been investigated in this country, confirming data from other parts of the world.

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2006-11-01
2024-03-29
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