RT Journal Article SR Electronic(1) A1 Gilmour, Matthew W. A1 Bernard, Kathryn A1 Tracz, Dobryan M. A1 Olson, Adam B. A1 Corbett, Cindi R. A1 Burdz, Tamara A1 Ng, Betty A1 Wiebe, Deborah A1 Broukhanski, George A1 Boleszczuk, Peter A1 Tang, Patrick A1 Jamieson, Frances A1 Van Domselaar, Gary A1 Plummer, Francis A. A1 Berry, Jody D.YR 2007 T1 Molecular typing of a Legionella pneumophila outbreak in Ontario, Canada JF Journal of Medical Microbiology, VO 56 IS 3 SP 336 OP 341 DO https://doi.org/10.1099/jmm.0.46738-0 PB Microbiology Society, SN 1473-5644, AB An outbreak of Legionnaires' disease at a long-term care facility in Ontario, Canada from September to October 2005 resulted in the death of 23 residents and the illness of 112 other people. In response, molecular methods were developed to detect Legionella pneumophila in clinical lung samples and to subtype isolates from clinical and environmental samples. The targeted genetic loci included Legionella-specific virulence determinants (mip, icmO, sidA and lidA) and core bacterial determinants (ftsZ, trpS and dnaX). An established amplified fragment length polymorphism typing method provided the first indication of genetic relatedness between strains recovered from clinical samples and strains cultured from environmental samples taken from the outbreak site. These associations were verified using the European Working Group for Legionella Infections sequence-based typing protocol targeting the flaA, pilE, asd, mip, mompS and proA loci. These molecular typing methods confirmed the outbreak source as a contaminated air conditioning cooling tower., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.46738-0