1887

Abstract

is a component of the indigenous microbiota but is also responsible for anaerobic infections of the gastrointestinal tract and oral cavity. The aim of the present study was to investigate the influence of oxidative stress on the pathogenicity of . Germ-free mice were challenged intraperitoneally with parental (wt) or oxidative stress adapted (aero) strains. Bacterial virulence was evaluated by histopathology, hyperaemia and blood analysis [C-reactive protein (CRP), serum albumin and white blood cells (WBCs)], 3 and 10 days after challenge. CRP levels and WBC count were higher in animals challenged with the aero strain, and the albumin level was lower in this group, only 10 days after infection (<0.05). Body weight gain was significantly reduced whereas hyperaemia and ratios of spleen/organ weight were increased in animals challenged with the aero strain (<0.05). The liver of animals challenged with the aero strain showed hyperaemia, vasodilatation as well as an increase in the number of inflammatory cells and liver/organ weight ratio (<0.05). Similar, but more discrete, alterations were observed in the small intestine of animals challenged with the aero strain. Studies on stress responses of this putative pathogen may help to better understand the aggressive potential and virulence markers of anaerobic bacteria.

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2007-03-01
2024-04-28
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References

  1. Arantes R. M., Nogueira A. M. 1997; Distribution of enteroglucagon and peptide YY-immunoreactive cells in the intestinal mucosa of germ-free and conventional mice. Cell Tissue Res 290:61–69 [CrossRef]
    [Google Scholar]
  2. Baumgartner J. C. Jr, Faulkler W. A., Beckerman T. 1992; Experimentally induced infection by oral anaerobic microorganisms in a mouse model. Oral Microbiol Immunol 7:253–256 [CrossRef]
    [Google Scholar]
  3. Cabral V. L. R., Carvalho L., Miszputen S. J. 2001; Importância da albumina sérica na avaliação nutricional e de atividade inflamatória em pacientes com doença de Crohn. Arq Gastroenterol 38:104–108
    [Google Scholar]
  4. Casadevall A., Pirofski L. 1999; Host-pathogen interactions: redefining the basic concepts of virulence and pathogenicity. Infect Immun 6:3703–3713
    [Google Scholar]
  5. Diniz C. G., Cara D. C., Rodrigues A. C. N. R., Nicoli J. R., Farias L. M., Carvalho M. A. R. 2000; Effect of metronidazole on the pathogenicity of resistant Bacteroides strains in gnotobiotic mice. Antimicrob Agents Chemother 44:2419–2423 [CrossRef]
    [Google Scholar]
  6. Diniz C. G., Arantes R. M., Cara D. C., Lima F. L., Nicoli J. R., Carvalho M. A. R., Farias L. M. 2003; Enhanced pathogenicity of susceptible strains of the Bacteroides fragilis group subjected to low doses of metronidazole. Microbes Infect 4:19–26
    [Google Scholar]
  7. Drabkin D. L., Austin J. H. 1932; Spectrophotometric constants for common haemoglobin derivates in human dog and rabbit blood. J Biol Chem 98:719–733
    [Google Scholar]
  8. Dunn D. L., Barke R. A., Knight N. B., Humphrey E. W., Simmons R. L. 1985; Role of resident macrophages, peripheral neutrophils, and trans-lymphatic absorption in bacterial clearance from the peritoneal cavity. Infect Immun 49:257–264
    [Google Scholar]
  9. Fey G. H., Gauldie J. 1990; The acute phase response of the liver in inflammation. Prog Liver Dis 9:89–116
    [Google Scholar]
  10. Ganusov V. V. 2003; Evolution of virulence: adaptative or not?. Trends Microbiol 11:112–113 [CrossRef]
    [Google Scholar]
  11. Huntley J. S., Kelly M. B. 2005; C-reactive protein: a valuable acute investigation. A case of pneumococcal meningitis presenting as ankle pain. Emerg Med J 22:602–603 [CrossRef]
    [Google Scholar]
  12. Imlay J. A. 2003; Pathways of oxidative damage. Annu Rev Microbiol 57:395–418 [CrossRef]
    [Google Scholar]
  13. Li X., Kolltveit K. M., Tronstad L., Olsen I. 2000; Systemic diseases caused by oral infection. Clin Microbiol Rev 13:547–558 [CrossRef]
    [Google Scholar]
  14. Mello S. T., Liberti E. A., Sant'Ana D. M. G., Neto M. H. M. 2004; Estudo morfoquantitativo do plexo mioentérico do duodeno de ratos submetidos à carência de proteínas e vitaminas do complexo B. Acta Scient Biol Sci 26:251–256
    [Google Scholar]
  15. National Research Council. 1996 Guide for the Care and Use of Laboratory Animals Washington, DC: National Academy Press;
    [Google Scholar]
  16. Nilsen T., Ghosh A. S., Goldeberg M. B., Young K. D. 2004; Branching sites and morphological abnormalities as ectopic poles in shape defective Escherichia coli . Mol Microbiol 52:1045–1054 [CrossRef]
    [Google Scholar]
  17. Orhue N. E. J., Nwanze E. A. C., Okafor A. 2005; Serum total protein, albumin and globulin levels in Trypanosoma brucei -infected rabbits: effects of orally administered Scoparia dulcis . Afr J Biotechnol 4:1152–1155
    [Google Scholar]
  18. Papet I., Dardevet D., Sornet C., Bechereau F., Prugnaud D. 2003; Acute phase protein levels and thymus, spleen and plasma protein synthesis rates differ in adult and old rats. J Nutr 33:215–219
    [Google Scholar]
  19. Patschkowski T., Bates D. M., Kiley P. J. 2000; Mechanisms for sensing and responding to oxygen deprivation. In Bacterial Stress Responses pp  61–78 Edited by Storz G., Hengge-Aronis R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  20. Pleasants J. R. 1974; Gnotobiotics. In Handbook of Laboratory Animal Science pp  119–174 Edited by Melby E. C. Jr, Altmann N. H. Cleveland: CRC Press;
    [Google Scholar]
  21. Rawlinson A., Duerden B. I., Goodwin L. 1993; Effects of root planing on the distribution of microorganisms adult periodontitis sites. Eur J Prosthodont Restor Dent 3:103–110
    [Google Scholar]
  22. Rocha E. R., Selby T., Coleman J. P., Smith C. J. 1996; Oxidative stress response in an anaerobe, Bacteroides fragilis : a role for catalase in protection against hydrogen peroxide. J Bacteriol 178:6895–6903
    [Google Scholar]
  23. Rocha E. R., Owens G. Jr, Smith C. J. 2000; The redox-sensitive transcriptional activator OxyR regulates the peroxide response regulon in the obligate anaerobe Bacteroides fragilis . J Bacteriol 182:5059–5069 [CrossRef]
    [Google Scholar]
  24. Sansonetti P. J. 2001; Phagocytosis of bacterial pathogens: implications in the host response. Semin Immunol 13:381–390 [CrossRef]
    [Google Scholar]
  25. Shah H. N., Gharbia S. E. 1992; Biochemical and chemical studies on strains designated Prevotella intermedia and proposal of a new pigmented species Prevotella nigrescens sp. Int J Syst Bacteriol 42:542–546 [CrossRef]
    [Google Scholar]
  26. Silva V. L., Diniz C. G., Cara D. C., Santos S. G., Nicoli J. R., Carvalho M. A. R., Farias L. M. 2005; Enhanced pathogenicity of Fusobacterium nucleatum adapted to oxidative stress. Microb Pathog 39:131–138 [CrossRef]
    [Google Scholar]
  27. Socransky S. S., Gibbons R. J. 1965; Required role of periodontal disease. J Infect Dis 115:247–253 [CrossRef]
    [Google Scholar]
  28. Socransky S. S., Haffajee A. D. 2005; Periodontal microbial ecology. Periodontology 2000; 38:135–187 [CrossRef]
    [Google Scholar]
  29. Tung C. B., Tung C. F., Yang D. Y., Hu W. H., Hung D. Z., Peng Y. C., Chang C. S. 2005; Extremely high levels of alkaline phosphatase in adult patients as a manifestation of bacteremia. Hepatogastroenterology 52:1347–1350
    [Google Scholar]
  30. Zheng M., Storz G. 2000; Oxidative stress. In Bacterial Stress Responses pp  47–59 Edited by Storz G, Hengge-Aronis R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
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