%0 Journal Article %A Han, Kyung Ho %A Choi, Seon Young %A Lee, Je Hee %A Lee, Hyejon %A Shin, Eun Hee %A Agtini, Magdarina D. %A von Seidlein, Lorenz %A Ochiai, R. Leon %A Clemens, John D. %A Wain, John %A Hahn, Ji-Sook %A Lee, Bok Kwon %A Song, Manki %A Chun, Jongsik %A Kim, Dong Wook %T Isolation of Salmonella enterica subspecies enterica serovar Paratyphi B dT+, or Salmonella Java, from Indonesia and alteration of the d-tartrate fermentation phenotype by disrupting the ORF STM 3356 %D 2006 %J Journal of Medical Microbiology, %V 55 %N 12 %P 1661-1665 %@ 1473-5644 %R https://doi.org/10.1099/jmm.0.46792-0 %K SGI1, Salmonella genomic island 1 %K MDR, multidrug-resistant %K MLST, multilocus sequence typing %I Microbiology Society, %X Salmonella enterica subspecies enterica serovar Paratyphi B [O1,4,(5),12 : Hb : 1,2] can cause either an enteric fever (paratyphoid fever) or self-limiting gastroenteritis in humans. The d-tartrate non-fermenting variant S. enterica subsp. enterica serovar Paratyphi B dT− (S. Paratyphi B) is the causative agent of paratyphoid fever, and the d-tartrate fermenting variant S. enterica subsp. enterica serovar Paratyphi B dT+ (S. Paratyphi B dT+; formerly called Salmonella Java) causes gastroenteritis. S. Java is currently recognized as an emerging problem worldwide. Twelve dT+ S. Java isolates were collected in Indonesia between 2000 and 2002. One-third of them contained Salmonella genomic island 1 (SGI1), which gives the multidrug-resistant phenotype to the bacteria. In this study, a PCR-based method to detect a single nucleotide difference responsible for the inability to ferment d-tartrate, reported elsewhere, was validated. The d-tartrate fermenting phenotype of S. Java was converted to the non-fermenting phenotype by the disruption of the ORF STM 3356, and the d-tartrate non-fermenting phenotype of the ORF STM 3356-disrupted strain and the dT− reference strain was changed to the dT+ phenotype by complementing ORF STM 3356 in trans. The results show that the dT+ phenotype requires a functional product encoded by STM 3356, and support the use of the PCR-based discrimination method for S. Paratyphi B and S. Java as the standard differentiation method. %U https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.46792-0