1887

Abstract

subspecies serovar Paratyphi B [O1,4,(5),12 : Hb : 1,2] can cause either an enteric fever (paratyphoid fever) or self-limiting gastroenteritis in humans. The -tartrate non-fermenting variant subsp. serovar Paratyphi B dT (. Paratyphi B) is the causative agent of paratyphoid fever, and the -tartrate fermenting variant subsp. serovar Paratyphi B dT (. Paratyphi B dT; formerly called Java) causes gastroenteritis. . Java is currently recognized as an emerging problem worldwide. Twelve dT . Java isolates were collected in Indonesia between 2000 and 2002. One-third of them contained genomic island 1 (SGI1), which gives the multidrug-resistant phenotype to the bacteria. In this study, a PCR-based method to detect a single nucleotide difference responsible for the inability to ferment -tartrate, reported elsewhere, was validated. The -tartrate fermenting phenotype of . Java was converted to the non-fermenting phenotype by the disruption of the ORF STM 3356, and the -tartrate non-fermenting phenotype of the ORF STM 3356-disrupted strain and the dT reference strain was changed to the dT phenotype by complementing ORF STM 3356 . The results show that the dT phenotype requires a functional product encoded by STM 3356, and support the use of the PCR-based discrimination method for . Paratyphi B and . Java as the standard differentiation method.

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2006-12-01
2024-03-28
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References

  1. Barker R. M. 1985; Utilization of d -tartaric acid by Salmonella Paratyphi B and Salmonella Java: comparison of anaerobic plate test, lead acetate test and turbidity test. J Hyg (Lond) 95:107–114 [CrossRef]
    [Google Scholar]
  2. Boyd D., Peters G. A., Cloeckaert A., Boumedine K. S., Chaslus-Dancla E., Imberechts H., Mulvey M. R. 2001; Complete nucleotide sequence of a 43-kilobase genomic island associated with the multidrug resistance region of Salmonella enterica serovar Typhimurium DT104 and its identification in phage type DT120 and serovar Agona. J Bacteriol 183:5725–5732 [CrossRef]
    [Google Scholar]
  3. Boyd D., Cloeckaert A., Chaslus-Dancla E., Mulvey M. R. 2002; Characterization of variant Salmonella genomic island 1 multidrug resistance regions from serovars Typhimurium DT104 and Agona. Antimicrob Agents Chemother 46:1714–1722 [CrossRef]
    [Google Scholar]
  4. Chart H. 2003; The pathogenicity of strains of Salmonella Paratyphi B and Salmonella Java. J Appl Microbiol 94:340–348 [CrossRef]
    [Google Scholar]
  5. Doublet B., Lailler R., Meunier D., Brisabois A., Boyd D., Mulvey M. R., Chaslus-Dancla E., Cloeckaert A. 2003; Variant Salmonella genomic island 1 antibiotic resistance gene cluster in Salmonella enterica serovar Albany. Emerg Infect Dis 9:585–591 [CrossRef]
    [Google Scholar]
  6. Doublet B., Boyd D., Mulvey M. R., Cloeckaert A. 2005; The Salmonella genomic island 1 is an integrative mobilizable element. Mol Microbiol 55:1911–1924 [CrossRef]
    [Google Scholar]
  7. Doublet B., Weill F. X., Fabre L., Chaslus-Dancla E., Cloeckaert A. 2004; Variant Salmonella genomic island 1 antibiotic resistance gene cluster containing a novel 3′-N-aminoglycoside acetyltransferase gene cassette, aac (3)- Id , in Salmonella enterica serovar Newport. Antimicrob Agents Chemother 48:3806–3812 [CrossRef]
    [Google Scholar]
  8. Goh Y. L., Yasin R., Puthucheary S. D., Koh Y. T., Lim V. K., Taib Z., Thong K. L. 2003; DNA fingerprinting of human isolates of Salmonella enterica serotype Paratyphi B in Malaysia. J Appl Microbiol 95:1134–1142 [CrossRef]
    [Google Scholar]
  9. Kauffmann F. 1955; Differential diagnosis and pathogenicity of Salmonella java and Salmonella paratyphi B . Z Hyg Infektionskr 141:546–550 (in German [CrossRef]
    [Google Scholar]
  10. Kim D. W., Lenzen G., Page A. L., Legrain P., Sansonetti P. J., Parsot C. 2005; The Shigella flexneri effector OspG interferes with innate immune responses by targeting ubiquitin-conjugating enzymes. Proc Natl Acad Sci U S A 102:14046–14051 [CrossRef]
    [Google Scholar]
  11. Malorny B., Bunge C., Helmuth R. 2003; Discrimination of d -tartrate-fermenting and -nonfermenting Salmonella enterica subsp. enterica isolates by genotypic and phenotypic methods. J Clin Microbiol 41:4292–4297 [CrossRef]
    [Google Scholar]
  12. Meunier D., Boyd D., Mulvey M. R., Baucheron S., Mammina C., Nastasi A., Chaslus-Dancla E., Cloeckaert A. 2002; Salmonella enterica serotype Typhimurium DT 104 antibiotic resistance genomic island I in serotype Paratyphi B. Emerg Infect Dis 8:430–433 [CrossRef]
    [Google Scholar]
  13. Miko A., Guerra B., Schroeter A., Dorn C., Helmuth R. 2002; Molecular characterization of multiresistant d -tartrate-positive Salmonella enterica serovar Paratyphi B isolates. J Clin Microbiol 40:3184–3191 [CrossRef]
    [Google Scholar]
  14. Mulvey M. R., Boyd D., Cloeckaert A., Ahmed R., Ng L. K. 2004; Emergence of multidrug-resistant Salmonella Paratyphi B dT+ . Canada. Emerg Infect Dis 10:1307–1310 [CrossRef]
    [Google Scholar]
  15. Prager R., Rabsch W., Streckel W., Voigt W., Tietze E., Tschape H. 2003; Molecular properties of Salmonella enterica serotype Paratyphi B distinguish between its systemic and its enteric pathovars. J Clin Microbiol 41:4270–4278 [CrossRef]
    [Google Scholar]
  16. Threlfall J., Levent B., Hopkins K. L., de Pinna E., Ward L. R., Brown D. J. 2005; Multidrug-resistant Salmonella Java. Emerg Infect Dis 11:170–171 [CrossRef]
    [Google Scholar]
  17. Tjaniadi P., Lesmana M., Subekti D. & 10 other authors; 2003; Antimicrobial resistance of bacterial pathogens associated with diarrheal patients in Indonesia. Am J Trop Med Hyg 68:666–670
    [Google Scholar]
  18. Weill F.-X., Fabre L., Grandry B., Grimont P. A. D., Casin I. 2005; Multiple-antibiotic resistance in Salmonella enterica serotype Paratyphi B isolates collected in France between 2000 and 2003 is due mainly to strains harboring Salmonella genomic islands 1, 1-B, and 1-C. Antimicrob Agents Chemother 49:2793–2801 [CrossRef]
    [Google Scholar]
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