Real-time TaqMan PCR for rapid detection and typing of genes encoding CTX-M extended-spectrum β-lactamases
Birkett, Christopher I. and Ludlam, Hugo A. and Woodford, Neil and Brown, Derek F. J. and Brown, Nicholas M. and Roberts, Mark T. M. and Milner, Nicola and Curran, Martin D.,, 56, 52-55 (2007),
doi = https://doi.org/10.1099/jmm.0.46909-0,
publicationName = Microbiology Society,
issn = 0022-2615,
abstract= The prevalence of CTX-M-producing members of the Enterobacteriaceae is increasing worldwide. A novel, multiplex, real-time TaqMan PCR assay to detect and type bla CTX-M genes is described which is an improvement on previously described techniques with respect to reduced assay time, elimination of the need for protracted post-PCR processing and the convenience of a single reaction vessel. Based on β-lactam antibiogram and MIC data, 478 of 1279 Enterobacteriaceae isolates from clinical blood and urine culture specimens were selected and tested for extended-spectrum β-lactamase (ESBL) production using phenotypic methods. The new TaqMan assay detected and typed bla CTX-M genes in 21 of 28 ESBL-producing isolates.,
language=,
type=