1887

Abstract

The prevalence of CTX-M-producing members of the is increasing worldwide. A novel, multiplex, real-time TaqMan PCR assay to detect and type genes is described which is an improvement on previously described techniques with respect to reduced assay time, elimination of the need for protracted post-PCR processing and the convenience of a single reaction vessel. Based on -lactam antibiogram and MIC data, 478 of 1279 isolates from clinical blood and urine culture specimens were selected and tested for extended-spectrum -lactamase (ESBL) production using phenotypic methods. The new TaqMan assay detected and typed genes in 21 of 28 ESBL-producing isolates.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.46909-0
2007-01-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/jmm/56/1/52.html?itemId=/content/journal/jmm/10.1099/jmm.0.46909-0&mimeType=html&fmt=ahah

References

  1. Bauernfeind A., Grimm H., Schweighart S. 1990; A new plasmidic cefotaximase in a clinical isolate of Escherichia coli . Infection 37:851–858
    [Google Scholar]
  2. Bonnet R. 2004; Growing group of extended-spectrum beta-lactamases: the CTX-M enzymes. Antimicrob Agents Chemother 48:1–14 [CrossRef]
    [Google Scholar]
  3. Carter M. W., Oakton K. J., Warner M., Livermore D. M. 2000; Detection of extended-spectrum beta-lactamases in klebsiellae with the Oxoid combination disk method. J Clin Microbiol 38:4228–4232
    [Google Scholar]
  4. Livermore D. M., Hawkey P. M. 2005; CTX-M: changing the face of ESBLs in the UK. J Antimicrob Chemother 56:451–454 [CrossRef]
    [Google Scholar]
  5. Narayanan S. K., Nagaraja T. G., Chengappa M. M., Stewart G. C. 2001; Cloning, sequencing and expression of the leukotoxin gene from Fusobacterium necrophorum . Infect Immun 69:5447–5455 [CrossRef]
    [Google Scholar]
  6. Paterson D. L., Bonomo R. A. 2005; Extended-spectrum β -lactamases: a clinical update. Clin Microbiol Rev 18:657–686 [CrossRef]
    [Google Scholar]
  7. Paterson D. L., Ko W. C., Von Gottberg A., Casellas J. M., Mulazimoglu L., Klugman K. P., Bonomo R. A., Rice L. B., McCormack J. G., Yu V. L. 2001; Outcome of cephalosporin treatment for serious infections due to apparently susceptible organisms producing extended-spectrum beta-lactamases: implications for the clinical microbiology laboratory. J Clin Microbiol 39:2206–2212 [CrossRef]
    [Google Scholar]
  8. Paterson D. L., Ko W. C., Von Gottberg A., Mohapatra S., Casellas J. M., Goossens H., Mulazimoglu L., Trenholme G., Klugman K. P. other authors 2004; International prospective study of Klebsiella pneumoniae bacteraemia: implications of extended-spectrum beta-lactamase production in nosocomial infections. Ann Intern Med 140:26–32 [CrossRef]
    [Google Scholar]
  9. Walther-Rasmussen J., Hoiby N. 2004; Cefotaximases (CTX-M-ases), an expanding family of extended-spectrum β -lactamases. Can J Microbiol 50:137–165 [CrossRef]
    [Google Scholar]
  10. Woodford N., Sundsfjord A. 2005; Molecular detection of antibiotic resistance: when and where?. J Antimicrob Chemother 56:259–261 [CrossRef]
    [Google Scholar]
  11. Woodford N., Ward M. E., Kaufmann M. E., Turton J., Fagan E. J., James D., Johnson A. P., Pike R., Warner M. other authors 2004; Community and hospital spread of Escherichia coli producing CTX-M extended-spectrum beta-lactamases in the UK. J Antimicrob Chemother 54:735–743 [CrossRef]
    [Google Scholar]
  12. Woodford N., Fagan E. J., Ellington M. J. 2006; Multiplex PCR for rapid detection of genes encoding CTX-M extended-spectrum β -lactamases. J Antimicrob Chemother 57:154–155
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.46909-0
Loading
/content/journal/jmm/10.1099/jmm.0.46909-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error