RT Journal Article SR Electronic(1) A1 Vankerckhoven, Vanessa A1 Moreillon, Philippe A1 Piu, Stéphane A1 Giddey, Marlyse A1 Huys, Geert A1 Vancanneyt, Marc A1 Goossens, Herman A1 Entenza, José M.YR 2007 T1 Infectivity of Lactobacillus rhamnosus and Lactobacillus paracasei isolates in a rat model of experimental endocarditis JF Journal of Medical Microbiology, VO 56 IS 8 SP 1017 OP 1024 DO https://doi.org/10.1099/jmm.0.46929-0 PB Microbiology Society, SN 1473-5644, AB The potential pathogenicity of selected (potentially) probiotic and clinical isolates of Lactobacillus rhamnosus and Lactobacillus paracasei was investigated in a rat model of experimental endocarditis. In addition, adhesion properties of the lactobacilli for fibrinogen, fibronectin, collagen and laminin, as well as the killing activity of the platelet-microbicidal proteins fibrinopeptide A (FP-A) and connective tissue activating peptide 3 (CTAP-3), were assessed. The 90 % infective dose (ID90) of the L. rhamnosus endocarditis isolates varied between 106 and 107 c.f.u., whereas four of the six (potentially) probiotic L. rhamnosus isolates showed an ID90 that was at least 10-fold higher (108 c.f.u.) (P<0.001). In contrast, the two other probiotic L. rhamnosus isolates exhibited an ID90 (106 and 107 c.f.u.) comparable to the ID90 of the clinical isolates of this species investigated (P>0.05). Importantly, these two probiotic isolates shared the same fluorescent amplified fragment length polymorphism cluster type as the clinical isolate showing the lowest ID90 (106 c.f.u.). L. paracasei tended to have a lower infectivity than L. rhamnosus (ID90 of 107 to ≥108 c.f.u.). All isolates had comparable bacterial counts in cardiac vegetations (P>0.05). Except for one L. paracasei strain adhering to all substrates, all tested lactobacilli adhered only weakly or not at all. The platelet peptide FP-A did not show any microbicidal activity against the tested lactobacilli, whereas CTAP-3 killed the majority of the isolates. In general, these results indicate that probiotic lactobacilli display a lower infectivity in experimental endocarditis compared with true endocarditis pathogens. However, the difference in infectivity between L. rhamnosus endocarditis and (potentially) probiotic isolates could not be explained by differences in adherence or platelet microbicidal protein susceptibility. Other disease-promoting factors may exist in these organisms and warrant further investigation., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.46929-0