1887

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Volume 56, Issue 5

The dynamics of spp., , and spp. in dental plaque biofilm as analysed by five-colour multiplex fluorescence hybridization Free

Abstract

The formation and composition of dental plaque biofilm are important factors which influence the development of gingivitis, caries and periodontitis. Studying dental plaque biofilm in models can cause an oversimplification of the real conditions in the oral cavity. In this study, bovine enamel slabs were fixed in an individual acrylic appliance to quantify dental plaque formation and composition using multiplex fluorescence hybridization (FISH) and confocal laser scanning microscopy. Each of the five oligonucleotide probes used for FISH was specific for either eubacteria or one of four frequently isolated bacterial constituents belonging to early and late colonizers of tooth surfaces. The thickness of formed biofilm increased from 14.9±5.0 μm after 1 day to 49.3±11.6 μm after 7 days. spp. were predominant in 1-day-old dental plaque and decreased significantly after 7 days (=0.0061). Compared to the first day, decreased after 2 days and increased significantly after 7 days (=0.0006). The decreases of content on day 2 and day 7 were significant (=0.0028). Changes in spp. were not significant during the study period (>0.05). The results showed that an observation period of 7 days was required to detect significant changes in spp. and . The multiplex FISH used is suitable for analysing the dynamics of four important bacterial constituents in the oral biofilm in epidemiological studies.

  • Received:
  • Accepted:
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Keyword(s): CLSM, confocal laser scanning microscopy and FISH, fluorescence in situ hybridization
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2007-05-01
2024-04-19
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The in vivo dynamics of Streptococcus spp., Actinomyces naeslundii, Fusobacterium nucleatum and Veillonella spp. in dental plaque biofilm as analysed by five-colour multiplex fluorescence in situ hybridization
J. Med. Microbiol. 56, 681 (2007); https://doi.org/10.1099/jmm.0.47094-0
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