1887

Abstract

The objective of this study was to investigate the sequence diversity in a single country of a family of integrative and conjugative elements (ICEs) that are vectors of antibiotic resistance in and , and test the hypothesis that they emerged from a single lineage. Sixty subjects aged 9 months – 13 years were recruited and oropharyngeal samples cultured. Up to 10 morphologically distinct spp. were purified, and then the species were determined and differentiated by partial sequence analysis of 16S rDNA and , respectively. ICEs were detected by PCR directed at five genes distributed evenly across the ICE. These amplicons were sequenced and aligned by the neighbour-joining algorithm. A total of 339 distinguishable isolates were cultured. ICEs with all 5 genes present were found in 9 of 110 (8 %) and 21 of 211 (10 %) , respectively. ICEs were not detected among the other . A total of 20 of 60 (33 %) children carried at least 1 oropharyngeal isolate with an ICE possessing all 5 genes. One of the five genes, integrase, however, consisted of two lineages, one of which was highly associated with . The topology of neighbour-joining trees of the remaining four ICE genes was compared and showed a lack of congruence; though, the genes form a common pool among and . This family of antibiotic resistance ICEs was prevalent among the children studied, was genetically diverse, formed a large gene pool, transferred between and , lacked population structure and possessed features suggestive of panmixia, all indicating it has not recently emerged from a single source.

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2007-06-01
2024-03-28
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References

  1. Barbour M. L., Booy R., Crook D. W., Griffiths H., Chapel H. M., Moxon E. R., Mayon-White D. 1993a; Haemophilus influenzae type b carriage and immunity four years after receiving the Haemophilus influenzae oligosaccharide - CRM197 (HbOC) conjugate vaccine. Pediatr Infect Dis J 12:478–484 [CrossRef]
    [Google Scholar]
  2. Barbour M. L., Crook D. W., Mayon-White R. T. 1993b; An improved antiserum agar method for detecting carriage of Haemophilus influenzae type b. Eur J Clin Microbiol Infect Dis 12:215–217 [CrossRef]
    [Google Scholar]
  3. Davies J. E. 1997; Origins, acquisition and dissemination of antibiotic resistance determinants. Ciba Found Symp 207:15–27 (discussion 27–35)
    [Google Scholar]
  4. D'Costa V. M., McGrann K. M., Hughes D. W., Wright G. D. 2006; Sampling the antibiotic resistome. Science 311:374–377 [CrossRef]
    [Google Scholar]
  5. Dimopoulou I. D., Jordens J. Z., Legakis N. J., Crook D. W. 1997; A molecular analysis of Greek and UK Haemophilus influenzae conjugative resistance plasmids. J Antimicrob Chemother 39:303–307 [CrossRef]
    [Google Scholar]
  6. Dimopoulou I. D., Russell J. E., Mohd-Zain Z., Herbert R., Crook D. W. 2002; Site-specific recombination with the chromosomal tRNALeu gene by the large conjugative Haemophilus resistance plasmid. Antimicrob Agents Chemother 46:1602–1603 [CrossRef]
    [Google Scholar]
  7. Elwell L. P., De Graaff J., Seibert D., Falkow S. 1975; Plasmid-linked ampicillin resistance in Haempohilus influenza type b. Infect Immun 12:404–410
    [Google Scholar]
  8. Elwell L. P., Saunders J. R., Richmond M. H., Falkow S. 1977; Relationships among some R plasmids found in Haemophilus influenzae . J Bacteriol 131:356–362
    [Google Scholar]
  9. Falush D., Wirth T., Linz B. other authors 2003; Traces of human migrations in Helicobacter pylori populations. Science 299:1582–1585 [CrossRef]
    [Google Scholar]
  10. Finch R. G. 1998; Antibiotic resistance. J Antimicrob Chemother 42:125–128 [CrossRef]
    [Google Scholar]
  11. Fleischmann R. D., Adams M. D., White O., Clayton R. A., Kirkness F. F., Kerlavage A. R., Bult C. J., Tomb J. F., Dougherty B. A. other authors 1995; Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science 269:496–512 [CrossRef]
    [Google Scholar]
  12. Gunn B. A., Woodall J. B., Jones J. F., Thornsberry C. 1974; Ampicillin-resistant Haemophilus influenzae . Lancet 2:845
    [Google Scholar]
  13. Harrison A., Dyer D. W., Gillaspy A., Ray W. C., Mungur R., Matthew B. C., Zhong H., Gipson J., Gipson M. other authors 2005; Genomic sequence of an otitis media isolate of nontypeable Haemophilus influenzae : comparative study with H. influenzae serotype d, strain KW20. J Bacteriol 187:4627–4636 [CrossRef]
    [Google Scholar]
  14. Jahn G., Laufs R., Kaulfers P. M., Kolenda H. 1979; Molecular nature of two Haemophilus influenzae R factors containing resistances and the multiple integration of drug resistance transposons. J Bacteriol 138:584–597
    [Google Scholar]
  15. Juhas M., Crook D. W., Dimopoulou I. D., Lunter G., Harding R. M., Ferguson D. J., Hood D. W. 2007; Novel type IV secretion system involved in propagation of genomic islands. J Bacteriol 189:761–771 [CrossRef]
    [Google Scholar]
  16. Kilian M., Schiott C. R. 1975; Haemophili and related bacteria in the human oral cavity. Arch Oral Biol 20:791–796 [CrossRef]
    [Google Scholar]
  17. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  18. Laufs R., Kaulfers P. M. 1977; Molecular characterization of a plasmid specifying ampicillin resistance and its relationship to other R factors from Haemophilus influenzae . J Gen Microbiol 103:277–286 [CrossRef]
    [Google Scholar]
  19. Laufs R., Riess F. C., Jahn G., Fock R., Kaulfers P. M. 1981; Origin of Haemophilus influenzae R factors. J Bacteriol 147:563–568
    [Google Scholar]
  20. Leaves N. I., Dimopoulou I., Hayes I. other authors 2000; Epidemiological studies of large resistance plasmids in Haemophilus . J Antimicrob Chemother 45:599–604 [CrossRef]
    [Google Scholar]
  21. Levy S. B. 2006; Mechanisms for resistance in soil. Science 312:529
    [Google Scholar]
  22. Mathies A. W. Jr. 1972; Penicillins in the treatment of bacterial meningitis. J R Coll Physicians Lond 6:139–146
    [Google Scholar]
  23. Meats E., Feil E. J., Stringer S., Cody A. J., Goldstein R., Kroll J. S., Popovic T., Spratt B. G. 2003; Characterization of encapsulated and noncapsulated Haemophilus influenzae and determination of phylogenetic relationships by multilocus sequence typing. J Clin Microbiol 41:1623–1636 [CrossRef]
    [Google Scholar]
  24. Mohd-Zain Z., Turner S. L., Cerdeno-Tarraga A. M., Lilley A. K., Inzana T. J., Duncan A. J., Harding R. M., Hood D. W., Peto T. E., Crook D. W. 2004; Transferable antibiotic resistance elements in Haemophilus influenzae share a common evolutionary origin with a diverse family of syntenic genomic islands. J Bacteriol 186:8114–8122 [CrossRef]
    [Google Scholar]
  25. Saunders J. R., Sykes R. B. 1977; Transfer of a plasmid-specified beta-lactamase gene from Haemophilus influenzae . Antimicrob Agents Chemother 11:339–344 [CrossRef]
    [Google Scholar]
  26. Stuy J. H. 1980; Chromosomally integrated conjugative plasmids are common in antibiotic-resistant Haemophilus influenzae . J Bacteriol 142:925–930
    [Google Scholar]
  27. Syriopoulou V., Scheifele D., Smith A. L., Perry P. M., Howie V. 1978; Increasing incidence of ampicillin resistance in Hemophilus influenzae . J Pediatr 92:889–892 [CrossRef]
    [Google Scholar]
  28. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions – specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  29. Williams R. 1997; Developments in monitoring of antimicrobial resistance. WHO Drug Inf 11:248–249
    [Google Scholar]
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