1887

Abstract

isolates are not homogeneous in their patterns of pathogenicity in animals and adhesion to epithelial cells. During this investigation, genotypic differences were observed between two samples of strain 18 yeast phase (Pb18) previously cultured many times, one taken before (Pb18a) and the other after (Pb18b) animal inoculation. Random amplified polymorphic DNA analysis using the primer OPJ4 distinguished Pb18b from Pb18a by one 308 bp DNA fragment, which after cloning and sequencing was shown to encode a polypeptide sequence homologous to the protein -adaptin. It is suggested, by comparison to other micro-organisms, that this protein might play an important role in the virulence of . This result demonstrates the influence of subculturing on the genotype of this organism.

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2007-07-01
2024-05-01
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References

  1. Andreotti P. F., Monteiro Da Silva J. L., Bailao A. M., Soares C. M., Benard G., Soares C. P., Mendes-Giannini M. J. S. 2005; Isolation and partial characterization of a 30 kDa adhesin from Paracoccidioides brasiliensis . Microbes Infect 7:875–881 [CrossRef]
    [Google Scholar]
  2. Boehm M., Bonifacino J. S. 2001; Adaptins: the final recount. Mol Biol Cell 12:2907–2920 [CrossRef]
    [Google Scholar]
  3. Brummer E., Restrepo A., Hanson L. H., Stevens D. A. 1990; Virulence of Paracoccidioides brasiliensis : the influence of in vitro passage and storage. Mycopathologia 109:13–18 [CrossRef]
    [Google Scholar]
  4. Calcagno A. M., Nino-Vega G., San-Blas F., San-Blas G. 1998; Geographic discrimination of Paracoccidioides brasiliensis strains by randomly amplified polymorphic DNA analysis. J Clin Microbiol 36:1733–1736
    [Google Scholar]
  5. Castaneda E., Brummer E., Pappagianis D., Stevens D. A. 1987; Chronic pulmonary and disseminated paracoccidioidomycosis in mice: quantitation of progression and chronicity. J Med Vet Mycol 25:377–387 [CrossRef]
    [Google Scholar]
  6. Felipe M. S., Andrade R. V., Petrofeza S. S., Maranhao A. Q., Torres F. A., Albuquerque P., Arraes F. B., Arruda M., Azevedo M. O. other authors 2003; Transcriptome characterization of the dimorphic and pathogenic fungus Paracoccidioides brasiliensis by EST analysis. Yeast 20:263–271 [CrossRef]
    [Google Scholar]
  7. Fusco Almeida A. M., Matsumoto M. T., Baeza L. C., de Oliveira e Silva R. B., Kleiner A. A. P., Melhem M. S. C., Mendes Giannini M. J. S. the Laboratory Group on Cryptococcosis 2007; Molecular typing and antifungal susceptibility of clinical sequential isolates of Cryptococcus neoformans from Sao Paulo State, Brazil. FEMS Yeast Res 7:152–164 [CrossRef]
    [Google Scholar]
  8. Gokool S. 2003; σ 1- and μ1-adaptin homologues of Leishmania mexicana are required for parasite survival in the infected host. J Biol Chem 278:29400–29409 [CrossRef]
    [Google Scholar]
  9. Kurokawa C. S., Lopes C. R., Sugizaki M. F., Kuramae E. E., Franco M. F., Peraçoli M. T. 2005; Virulence profile of ten Paracoccidioides brasiliensis isolates: association with morphologic and genetic patterns. Rev Inst Med Trop Sao Paulo 47:257–262 [CrossRef]
    [Google Scholar]
  10. Lasker B. A., Page L. S., Lott T. J., Kobayashi G. S. 1992; Isolation, characterization, and sequencing of Candida albicans repetitive element 2. Gene 116:51–57 [CrossRef]
    [Google Scholar]
  11. Molinari-Madlum E. E. W. I., Felipe M. S. S., Soares C. M. A. 1999; Virulence of Paracoccidoides brasiliensis isolates can be correlated to groups defined by random amplified polymorphic DNA analysis. Med Mycol 37:269–276 [CrossRef]
    [Google Scholar]
  12. Motta T. R., Moreira-Filho C. A., Mendes R. P., Souza L. R., Sugizak M. F., Baueb S., Calich V. L., Vaz C. A. 2002; Evaluation of DNA polymorphisms amplified by arbitrary primers (RAPD) as genetically associated elements to differentiate virulent and non-virulent Paracoccidioides brasiliensis isolates. FEMS Immunol Med Microbiol 33:151–157 [CrossRef]
    [Google Scholar]
  13. Munn A. L. 2001; Molecular requirements for the internalization step of endocytosis: insights from yeast. Biochim Biophys Acta 1535236–257 [CrossRef]
    [Google Scholar]
  14. Oliveira A. A., Alves V. S., Moreira E. S. A., Correa A. Jr, Cisalpino P. S. 2002; Genetic polymorphism of Paracoccidioides brasiliensis by PFGE and RAPD. Annu Rev Biomed Sci 4: (special issue) 5
    [Google Scholar]
  15. Soares C. M., Madlun E. E., Da Silva S. P., Pereira M., Felipe M. S. 1995; Characterization of Paracoccidioides brasiliensis isolates by random amplified polymorphic DNA analysis. J Clin Microbiol 33:505–507
    [Google Scholar]
  16. Tan H., Andrews N. W. 2002; Don't bother to knock – the cell invasion strategy of Trypanosoma cruzi. . Trends Parasitol 18:427–428 [CrossRef]
    [Google Scholar]
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