1887

Abstract

Trypanosomes were observed in a peripheral blood smear from a 45-day-old Thai infant displaying fever, anaemia, cough and anorexia. Human trypanosomiasis is not endemic to Thailand, so parasite identification was undertaken to determine likely sources of the infection. Several morphological parameters of the trypanosomes were similar to those of and statistically different from those of -like parasites from a naturally infected indigenous rat. However, duplicate PCR assays with primers flanking trypanosome rRNA internal transcribed spacer 1 (ITS1) resulted in amplicons of ~623 bp that corresponded to the expected size for -like parasites. The ITS1 sequence from the infant's blood was 98 and 49 % identical to and sequences, respectively. Based on molecular results, it was concluded that the infant was infected with a -like () species.

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2007-08-01
2024-03-29
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