1887

Abstract

O1 serotype Ogawa and serotype Inaba isolates from the cholera epidemic that occurred in 2001 and 2002 in South Africa were compared with isolates of O1 serotype Inaba from the epidemic that occurred between 1980 and 1987. PFGE using I digestion was used to compare stored isolates received during the 1980s epidemic with those received during the epidemic in 2001/2002. A selected number of these isolates were then sequenced to compare the sequence of the gene in the O1 Ogawa strains of 2001/2002 with that in the O1 Inaba strains of the 1980s and 2001/2002. Isolates from the recent epidemic were shown to be related, irrespective of serotype, and had comparable banding patterns on PFGE, using I. They were distinctly different from those from the previous epidemic. Sequencing of the gene showed that the gene was highly conserved between the two epidemics. A single deletional mutation of an adenine residue was observed in the serotype Inaba isolates from the 2001/2002 epidemic, resulting in the serotype switch between the O1 strains from the recent epidemic. The distinct differences in PFGE patterns among isolates from the first and second epidemics exclude the possibility that the Inaba strain from the 1980s became dormant in the environment and mutated to serotype Ogawa, causing the 2001/2002 epidemic, despite the apparent consistency in the site of mutation in the Inaba serotypes between the two epidemics.

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2007-12-01
2024-04-18
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References

  1. Colwell R. R., Spira W. M. 1992; The ecology of Vibrio cholerae . In Cholera pp 107–127 Edited by Barua D., Greenough W. B. III New York: Plenum;
    [Google Scholar]
  2. Dalsgaard A., Forslund A., Sandvang D., Arntzen L., Keddy K. 2001; Vibrio cholerae O1 outbreak isolates in Mozambique in 1998 are multiple drug resistant, contain the SXT element and the aadA2 gene located on class 1 integrons. J Antimicrob Chemother 48:827–838 [CrossRef]
    [Google Scholar]
  3. Garg P., Nandy R. J., Chaudury P., Chowdhury N. R., De K., Ramamurthy T., Yamasaki S., Bhattacharya S. K., Takeda Y., Nair G. B. 2000; Emergence of Vibrio cholerae O1 biotype El Tor serotype Inaba from the prevailing O1 Ogawa serotype strains in India. J Clin Microbiol 38:4249–4253
    [Google Scholar]
  4. Ito T., Hiramatsu K., Ohshita Y., Yokota T. 1993; Mutations in the rfbT gene are responsible for the Ogawa to Inaba serotype conversion in Vibrio cholerae O1. Microbiol Immunol 37:281–288 [CrossRef]
    [Google Scholar]
  5. Küstner H. G. V., Du Plessis G. 1991; The cholera epidemic in South Africa, 1980–1987. Epidemiological features. S Afr Med J 79:539–544
    [Google Scholar]
  6. Lucas M. E. S., Deen J. L., von Seidlen L., Wang X.-Y., Ampuero J., Puri M., Ali M., Ansaruzzaman M., Amos J. other authors 2005; Effectiveness of mass oral cholera vaccination in Beira, Mozambique. N Engl J Med 352:757–767 [CrossRef]
    [Google Scholar]
  7. Manning P. A., Stroeher U. H., Morona R. 1994; Molecular basis for O-antigen biosynthesis in Vibrio cholerae O1: Ogawa-Inaba switching. . In Vibrio cholerae and Cholera: Molecular to Global Perspectives pp 77–92 Edited by Wachsmuth I. K., Blake P. A, Olsvik Ø. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  8. Mekalanos J. J., Swartz D. J., Pearson G. D., Harford N., Groyne F., de Wilde M. 1983; Cholera toxin genes: nucleotide sequence, deletion analysis and vaccine development. Nature 306:551–557 [CrossRef]
    [Google Scholar]
  9. Reeves P. R., Hobbs M., Valvano M. A., Skurnik M., Whitfield C., Coplin D., Kido N., Klena J., Maskell D. other authors 1996; Bacterial polysaccharide synthesis and gene nomenclature. Trends Microbiol 4:495–503 [CrossRef]
    [Google Scholar]
  10. Sack R. B., Miller C. E. 1969; Progressive changes of Vibrio serotypes in germ-free mice infected with Vibrio cholerae . J Bacteriol 99:688–695
    [Google Scholar]
  11. Sheehy T. W., Sprinz H., Augurson W. S., Formal S. B. 1966; Laboratory Vibrio cholerae infection in the United States. JAMA 197:321–326 [CrossRef]
    [Google Scholar]
  12. Stroeher U. H., Karageorgos L. E., Morona R., Manning P. A. 1992; Serotype conversion in Vibrio cholerae O1. Proc Natl Acad Sci U S A 89:2566–2570 [CrossRef]
    [Google Scholar]
  13. Swerdlow D. L., Isaäcson M. 1994; The epidemiology of cholera in Africa. In Vibrio cholerae and Cholera: Molecular to Global Perspectives . pp 297–307 Edited by Wachsmuth I. K., Blake P. A, Olsvik Ø. Washington, DC: American Society for Microbiology;
  14. Wachsmuth I. K., Evins G. M., Fields P., Olvik O., Popovic T., Bopp C. A., Wells J. G., Carillo C., Blake P. A. 1993; The molecular epidemiology of cholera in Latin America. J Infect Dis 167:621–626 [CrossRef]
    [Google Scholar]
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